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MetaMorph NX 2.5 Software

Photonics.com
Sep 2012
Molecular Devices LLCRequest Info
 
SUNNYVALE, Calif., Sept. 25, 2012 — Molecular Devices LLC has released version 2.5 of its MetaMorph NX microscopy automation and image analysis software, which now includes modules for the study of neuronal morphology and fiber structures, support for targeted illumination devices, and a high-speed image acquisition mode.

The Neurite Tracing application module simplifies the tasks associated with neuron research, while the Fiber Tracing application module quickly makes sense of complex, interwoven fiber structures. Added support for targeted illumination devices enables fast synchronization with regions of interest or an exact position in the image, and the new high-speed image acquisition mode allows researchers to record unlimited numbers of rapid changes in cellular function and behavior.

The software provides a single tool for researchers to use images of any size to rapidly produce quantitative and comparative descriptions of neuronal anatomy. The Sholl analysis, dendritic spine measurements and nuclei detection features add to the ability to investigate neurites. Color-coded segmentation overlays delineate intricate details of elaborate neuronal networks, including cell bodies, outgrowths, branch points and spines.

The Fiber Tracing application module uses a simple interface with powerful algorithms to differentiate fibers that cross over each other from fibers that branch from one another, quickly making sense of the entangled webs inherent to most fiber structures. The module supports applications including the study of elongated organisms, such as Caenorhabditis elegans and bacteria, cytoskeleton investigations and angiogenesis analyses.

Version 2.5 offers a direct interface to Andor Technology’s Mosaic and FRAPPA targeted illumination devices. A calibration wizard and ribbon-based work flow lead researchers from setup through to completion of fluorescence recovery after photobleaching, Förster resonance energy transfer, photoactivation, photobleaching and uncaging of caged compounds.

For more information, visit: metamorph.moleculardevices.com


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GLOSSARY
photobleaching
A process that helps optical fibers recover from damage induced by radiation. When silica is irradiated, bonds break and attenuation increases. Light in the fiber assists in recombining the species released by the broken bonds, decreasing attenuation.
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