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MicroSpec Microscope Interface

Princeton Instruments

TRENTON, N.J., Feb. 13, 2013 — Princeton Instruments is offering a microscope interface designed for exclusive use with the company’s aberration-free IsoPlane imaging spectrograph. The MicroSpec microscope interface allows researchers to easily acquire quality images and spectra of their samples with one pre-engineered system.

It attaches to a UDP port on select Olympus, Nikon and Zeiss inverted microscopes. The interface is not compatible with any other spectrographs, nor with upright microscopes. It supports all of the company’s cameras with a spectroscopy mount and without an internal shutter, and captures images with up to a 12 × 12-mm field of view.

The IsoPlane SCT 320 spectrograph provides high-quality imaging and spectra across the focal plane due to zero astigmatism and negligible spherical aberration and coma. The lack of aberrations also means more photons hit fewer pixels, increasing the signal-to-noise ratio of spectra.

When coupled to a microscope, the IsoPlane can resolve up to 25 line pairs per millimeter, the Nyquist limit of CCD cameras with 20-μm pixels. It can be used from the vacuum-UV to the mid-IR, with software-controlled scanning and an interchangeable triple grating turret. It also supports the proprietary IntelliCal state-of-the-art wavelength and intensity calibration package.

Applications of the MicroSpec-IsoPlane system include fluorescence, Raman, photoluminescence, single-molecule spectroscopy, quantum dots, live-cell imaging, and carbon nanotube short-wavelength infrared imaging and sum frequency generation.

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