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  • High-Power Dual Wavelength Laser for Multiphoton Microscopy

Coherent Inc.Request Info
Chameleon Discovery from CoherentThe Chameleon Discovery is a dual-wavelength ultrafast laser from Coherent. It combines the industry’s widest tuning range, highest power and shortest pulsewidth to enable cutting edge fluorescence detection techniques in non-linear microscopy. The primary output of the Chameleon Discovery is tunable from 680 nm – 1300 nm, with power up to 1.4 watts, high beam quality (M2 < 1.2), and short pulsewidth (100 fs). Plus an internal Group Velocity Dispersion (GVD) pre-compensator enables the shortest possible pulsewidth at the sample. The laser can simultaneously provide 1.5 watts of output at 1040 nm in a separate beam that also features short pulsewidth and high beam quality. In combination with its industrial-grade reliability and operational simplicity, this makes the Discovery ideal for use in the most demanding applications, including optogenetics and multi-modal imaging, and as a shared resource in a multi-user laser microscopy facility.

Discovery enables a broader range of non-linear applications than any other ultrafast laser source. The wide wavelength tuning, high power and short pulses of the primary output can excite all popular fluorophores (inclusive of eGFP, YFP and all the red-shifted proteins). Discovery’s secondary output at 1040 nm provides high power, short pulses (160 fs) and excellent beam parameters, enabling multiwavelength excitation of multiple probes and/or photoactivation of opsins for optogenetics applications. These include experiments based on established schemes using longer wavelength opsins like C1V1, as well as alternative wavelength pairings based on red-shifted calcium indicators of the GCaMP family currently under development. Moreover, the availability of phase-correlated dual wavelength pulses makes Chameleon Discovery also a perfect source for techniques like CARS and SRS.

Discovery is the newest member of the Coherent family of Chameleon lasers. Based on the company’s “industrial revolution in scientific lasers,” these compact lasers combine best in class performance with rugged (24/7) industrial-grade reliability and longevity, to deliver increased research productivity.


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