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The combination of atomic force microscopy (AFM) with time-resolved single-molecule-sensitive fluorescence microscopy in a single instrument opens up new avenues for fascinating investigations into the structure, dynamics, and interactions of single molecules or their assemblies in cells or tissue samples. The time-resolved microscopy platform MicroTime 200 from PicoQuant can be interfaced with a series of AFMs from Bruker, JPK, and Asylum Research while maintaining its full range of capabilities, such as super-resolution via Stimulated Emission Depletion (STED) or performing Fluorescence Lifetime Imaging (FLIM),. The AFM part reveals structural information of macromolecular complexes on the nanometer scale, while fluorescence lifetime data facilitates the identification of their constituent parts.
- Super-resolution and AFM Imaging
The integration of both techniques in a single instrument opens up new perspectives for applications in material and life science. Potential applications include live cell imaging, where the effect of protein changes on cell shape and structure can be investigated, merging of sub-nanometer AFM topographic imaging with optical encoded functionality or investigating inter- and intramolecular distances using force spectroscopy. An alternative application in material science is the study of the relationships between luminescence behavior and substrate shape in all kinds of biomaterials.
Thanks to seamless communication and state-of-the-art synchronization between the AFM and time-resolved fluorescence microscope, you can obtain superior AFM data without sacrificing optical image quality from the same sample area with a single instrument.