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Microscopy White Papers
Angle-Resolved Polarized Raman Microscopy for Determining the Orientation of Carbon Nanotubes
Determining the orientation of carbon nanotubes can expose crucial structural information about the material, which can help explain its properties and potential applications. A technique used to investigate this is angle-resolved polarized Raman microscopy. Carbon nanotube architectures are valuable in several technologies because of their attractive properties. Raman spectroscopy can analyze many properties of carbon nanotubes, including strain, diameter, and defects. Angle-resolved polarized...
Multi-Bandpass Filters for Fluorescence
Learn how multi-bandpass filters combined with multiple LEDs can enable versatile and compact fluorescence instrumentation.
Cooling SWIR Sensors: an Overview
Dark current is a critical parameter when one is looking to acquire a scientific imaging camera, especially in the short-wave infrared (SWIR) region. Very careful attention must be paid to the cooling method used to optimize this parameter. Multiple...
Dual Inverted Selective Plane Illumination Microscope
An overview of diSPIM
Overview of Photon etc’s Laser Line Tunable Filter (LLTF) and Tunable Laser Source (TLS) applications
The Laser Line Tunable Filter (LLTF) is a non-dispersive, widely tunable bandpass optical filter. At its core lies a volume Bragg grating (VBG) which efficiently selects and filters all but a narrow spectral window from a broadband light source. The...
FTP-2000 Focusing Translation Platform
Overview of FTP-2000.
Rapid Automated Modular Microscope
Overview of Rapid Automated Modular Microscope.
An Overview of CRISP Autofocus System
Continuous Reflective Interface Sample Placement (CRISP) system is designed to maintain focus over time, i.e. to compensate for thermal and other factors that may cause the sample to drift out of focus.
Dual Selective Plane Illumination Microscopy for Cleared Tissue
ct-dSPIM is a flexible and easy-to-use implementation of Selective Plane Illumination Microscopy (SPIM) that allows for dual views (d) of large samples such as cleared tissue (ct). It is one of many light sheet microscope configurations possible...
Laser Light Engine Output Stability
In this white paper, Dr. Iain Johnson, Lumencor’s Director of Technical Support, tests Lumencor’s laser-based CELESTA Light Engine for consistency, power, and stability using continuous wave illumination, pulsed illumination, and time-lapse imaging....
Single-Objective Light Sheet
An overview of the Single-Objective Light Sheet.
Spectral, Spatial and Temporal Optimization of Solid-State Light Engine Output
Specifications for illumination systems for biomedical imaging and industrial metrology generally converge on spectral, spatial and temporal light output characteristics. This article describes some of the ways in which solid-state Light Engines...
Conceptualizing Super-Resolution Microscopy eBook
A clever way to overcome the limitation caused by diffraction is to use 'pointillism strategy', a concept introduced by Rainer Heintzmann in 2005. The idea is to have only one fluorescent emitter (fluorophore) present at a given time for a given...
Exploring Microscopy Techniques eBook
With the evolution of microscopy systems, there has been a concurrent development in microscopy techniques. The first techniques in microscopy were based on Transmitted Light Microscopy. Additional techniques that are paramount to modern...
How to Price Your Confocal Microscopy System
Many factors affect the price of confocal microscopy systems and when purchasing a microscope, one must consider the complexity of the instrumentation and the capabilities it provides. Clearly the more complex the confocal, spinning disk, widefield...
Why Your Next Confocal Should Be Dragonfly
The Dragonfly naturally benefits from Andor EMCCD and sCMOS technologies, with motorized optical zoom, the system delivers industry-leading signal to noise and image fidelity. Applications ranging from single molecule to live cell confocal, TIRFM to...
Block Engineering's Tunable Mid-IR Quantum Cascade Lasers
Block Engineering's widely tunable mid-infrared Quantum Cascade Lasers are designed for use by researchers and manufacturers. Our next generation design features a six-fold increase in laser power, orders of magnitude better thermal and temporal...
Clean, New Bioenergy Sources: One Molecule at a Time
Researchers at Penn State University are spearheading a multidisciplinary research effort to explore ways to produce bioethanol more efficiently from lignocellulose. Importantly, this is not corn ethanol; it is ethanol derived from corn stalks,...
Raman Spectroscopy
To achieve accurate and precise Raman measurements, optical filters play a crucial role in enhancing signal-to-noise ratio, and reducing background interference. This white paper explores the fundamentals of optical filters, their types, and their...
Resonant Probe AFM: Uses and Advantages
A resonant probe atomic force microscope (AFM) offers capabilities and benefits not found in commonly used optical deflection AFMs in the measurement of physical constants, physical properties, and surface morphology. This article discusses resonant...
“Fingerprint” vs Handheld Raman Applications and the Different Optical Filters That Enable Them
Raman spectroscopy is a powerful non-invasive, non-contact tool capable of identifying molecular constituents of samples. Wavelength selective optical filters are used to block the dominant Rayleigh scattered light and transmit only the Raman...
Is a DIY Microscope for You?
For some advanced microscopy and nanoscopy techniques it becomes necessary to modify existing instrumentation or build an instrument from scratch. This article helps readers determine whether a do-it-yourself (DIY) microscope is the appropriate...
Sub 50 Femtosecond Pulse Lasers for Gentler Multiphoton Microscopy
Finding the ideal ultra-fast laser source for a multiphoton imaging setup is not trivial. It is a fine balance between peak power, pulse energy and laser wavelength. In this white paper we show that the use of sub 50 fs pulsed lasers produces very...
FISBA READYBeam™ - Compact Powerful Laser Source
The FISBA READYBeam™ is our answer to customers' increasing demand for compact, powerful, and reliable multicolor laser source that can be easily integrated into existing set-ups. The multicolor laser module is available in four main configurations...
Innovating the Tunable Light Source - How the Tunable Laser System is Applied in Fluorescence Microscopy
The market for the tunable light source is ever expanding due to its uses in countless fields, such as the inspection industry, machine visioning, and a wide range of optical experiments. Spectrolight Inc. has recently announced the Tunable Laser...
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April 2024
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