Cell signaling

Reporting on cell signaling is the main topic of Issue 21 of Cell Notes, a serial publication of !%Promega Corp.%! The 28-page document features articles on DNA purification using the PureYield plasmid miniprep systems, on selecting a cell-free protein expression system and on new bioluminescent cell-based assays to measure proteasome protease activities. It includes discussions on genetic reporters, luciferase reporter assays, choosing the right assay to monitor a signal transduction pathway, and an optimized system for studying transcriptional and post-transcriptional gene regulation. Reviews of recently published papers using P450-Glo and Beta-Glo assays and Halotag technology are included.