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Spectral Methods for Biological Analysis

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Electron-multiplying CCD cameras offer a new approach to quantitative biological microscopy.

Karl Garsha, Photometrics

An exciting development in light microscopy is the increasing availability of signal detection systems that record high-resolution emission spectra at each pixel in an image. These systems can be used for biological applications such as distinguishing the identities and relative contributions of fluorochromes with overlapping emission profiles.1 The image of a sample is shown using a conventional bandpass filter for red emission (A). The emission spectra of the fluorescent probes exhibit a high degree of overlap, making it difficult to discern protein localization in the context of cell...Read full article

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    Published: August 2007
    biological applicationsFeatureslight microscopyMicroscopysignal detection systems

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