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AFM reads genetic code

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Novel method could quantify gene expression in a single cell

David L. Shenkenberg

Gene expression can vary greatly among individual cells in the same tissue, especially in the case of cancer cells. Although technologies exist for quantifying gene expression in tissues, including DNA microarrays and reverse-transcription polymerase chain reaction (PCR), these technologies cannot quantify gene expression in a single cell. DNA microarrays and reverse-transcription PCR measure gene expression by quantifying messenger RNA binding to labeled DNA probes. The probes often contain a fluorophore and a quencher, such that messenger RNA binding releases the quencher and triggers a...Read full article

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    Published: March 2008
    Basic ScienceBiophotonicscellsDNAMicroscopyResearch & Technologyreverse-transcription polymerase chain reaction

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