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Get the Most from Förster Resonance Energy Transfer

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Use the best FRET method for your application.

Dr. Chris Baumann, MAG Biosystems

Förster resonance energy transfer (FRET) has become a common technique in fluorescence microscopy. For FRET to occur, two fluorochromes must be within a defined distance of one another (typically <10 nm) and have their dipoles correctly oriented. Under these conditions, when one fluorochrome (donor) is excited, its excited-state energy can be transferred to the second fluorochrome (acceptor) via a nonradiative transfer. Under ideal conditions — which rarely, if ever, exist — the excitation will selectively excite the donor, and the emission profiles of the donor and acceptor will not...Read full article

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    Published: August 2008
    Glossary
    fluorescence microscopy
    Fluorescence microscopy is a specialized optical imaging technique used in biology, chemistry, and materials science to visualize and study specimens that exhibit fluorescence. Fluorescence is the phenomenon where a substance absorbs light at one wavelength and emits light at a longer wavelength. In fluorescence microscopy, fluorescent dyes or proteins are used to label specific structures or molecules within a sample. The basic principles of fluorescence microscopy involve illuminating the...
    fluorochrome
    The combination of the organic dye in a stained specimen and the antibodies produced that is detected by exposure to light.
    förster resonance energy transfer
    Förster resonance energy transfer (FRET) is a mechanism describing the transfer of energy between two closely spaced fluorescent molecules. This phenomenon is named after the German scientist Theodor Förster, who first described it in the context of dipole-dipole interactions between molecules. In FRET, two fluorophores (molecules that fluoresce, or emit light, upon excitation) are involved: a donor and an acceptor. The donor fluorophore absorbs a photon and, instead of emitting a...
    BiophotonicsenergyFeaturesfluorescence microscopyfluorochromeFörster resonance energy transferMicroscopySensors & Detectors

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