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Confocal ratiometric imaging reveals reason for poor chromatography separations

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David L. Shenkenberg

Whether you are a biologist, biochemist, physician or pharmaceutical developer, chances are that you have wanted to know the chemical composition of a sample. One of the most popular ways to make this determination is reversed-phase liquid chromatography, which separates compounds into bands for identification. However, chromatography bands can become broad and even smear together, which hinders interpretation. Reversed-phase liquid chromatography is necessary not only for determining the composition of samples but also for separating molecules. For example, the pharmaceutical industry...Read full article

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    Published: October 2007
    Basic SciencebiochemistbiologistBiophotonicschemicalMicroscopyResearch & Technology

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