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Fluorescence flow cytometry monitors gene expression in time and space

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David L. Shenkenberg

If you look at an unfinished painting, you might wonder what the artist was trying to express before he set aside his paintbrush. Techniques such as fluorescence microscopy and microarray analysis can provide information about gene expression, but neither provides a complete picture. High-throughput fluorescence profiling using flow cytometry is a complementary approach that enables analysis of entire gene expression patterns within small organisms. Therefore, researchers at Dana-Farber Cancer Institute in Boston have used the method to observe gene expression in time and in space in...Read full article

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    Published: August 2007
    Glossary
    fluorescence microscopy
    Fluorescence microscopy is a specialized optical imaging technique used in biology, chemistry, and materials science to visualize and study specimens that exhibit fluorescence. Fluorescence is the phenomenon where a substance absorbs light at one wavelength and emits light at a longer wavelength. In fluorescence microscopy, fluorescent dyes or proteins are used to label specific structures or molecules within a sample. The basic principles of fluorescence microscopy involve illuminating the...
    Basic ScienceBiophotonicsfluorescence microscopymicroarray analysisMicroscopyNews & Featuresorganisms

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