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Chasing biochemicals in 3-D within living cells

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David L. Shenkenberg

Unfortunately, light behaves badly in living cells — scattering frantically and going to the wrong pigments, where it becomes absorbed. This unruly behavior makes it hard to follow biochemicals in cells, especially in 3-D. Elaborate setups with several cameras, mirrors and beamsplitters have been used for high-resolution 3-D tracking, but now researchers at the University of Illinois at Urbana-Champaign have used a simpler setup to monitor motor proteins and melanin in 3-D in living cells. To do 3-D tracking, the researchers combined basic in-focus microscopy in 2-D with defocused...Read full article

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    Published: August 2007
    Glossary
    light
    Electromagnetic radiation detectable by the eye, ranging in wavelength from about 400 to 750 nm. In photonic applications light can be considered to cover the nonvisible portion of the spectrum which includes the ultraviolet and the infrared.
    biochemicalsBiophotonicscameraschemicalslightMicroscopymirrorsNews & Features

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