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LEDs offer lighting alternative for DIC microscopy

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Gary Boas

Introduced in 1981, video-enhanced differential interference contrast (DIC) provides the ability to visualize subresolution phase objects such as single 25-nm-diameter microtubules. These organelles serve as tracks in cells, along which motor proteins move to transport cargo. In the early years of the technique, researchers struggled with the question of how to find a stable and uniform light source that had a narrow bandwidth. A fiber optic light scrambler introduced in 1985 addressed this issue by transforming the nonuniform light emission from an arc lamp into a uniform light...Read full article

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    Published: May 2007
    Glossary
    light
    Electromagnetic radiation detectable by the eye, ranging in wavelength from about 400 to 750 nm. In photonic applications light can be considered to cover the nonvisible portion of the spectrum which includes the ultraviolet and the infrared.
    BiophotonicscellsConsumerfiber opticslightMicroscopyResearch & TechnologyLEDs

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