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Exploring light and dark with two-photon microscopy

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Technique offers insight into the behavior of cells in the immune response

Gary Boas

Immunologists have long been interested in germinal centers, specialized structures that develop as part of the immune response. In the classical view of these structures, antigen-specific cells divide and mutate their receptors in one region — known as the “dark zone” — and then move into another — known as the “light zone” — to test the affinity of antibodies to a specific antigen. The rule is thought to be “survival of the fittest” because the B cells with highest affinity antibodies continue to multiply, whereas B cells that make the same or lower affinity antibodies drop...Read full article

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    Published: April 2007
    antibodiesBiophotonicsImmunologistsMicroscopyResearch & Technologytwo-photon microscopy

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