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Microfluidics and cylinder lens system counts molecules in a single cell

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Kevin Robinson

Researchers have developed a microfluidic system for counting individual protein molecules in cells. The method captures individual cells, lyses them, labels their contents and detects them individually with fluorescence microscopy. Developed by Richard N. Zare of Stanford University in California and his colleagues there and at Carnegie Institution, also in Stanford, the technique avoids the pitfalls of ensemble imaging and is particularly useful for studies where there are fewer than 1000 molecules of a particular protein of interest. Although single-molecule analysis can be done in...Read full article

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    Published: February 2007
    Glossary
    microfluidics
    Microfluidics is a multidisciplinary field that involves the manipulation and control of very small fluid volumes, typically in the microliter (10^(-6) liters) to picoliter (10^(-12) liters) range, within channels or devices with dimensions on the microscale. It integrates principles from physics, chemistry, engineering, and biotechnology to design and fabricate systems that handle and analyze fluids at the micro-level. Key features and aspects of microfluidics include: Miniaturization:...
    Biophotonicscylinder lens systemmicrofluidicsMicroscopymoleculesNews & Featuressingle cell

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