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Cell Cultures in 3-D

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Optical techniques contribute to advances in tissue engineering

Gary Boas, News Editor, [email protected]

Biologists have employed cell cultures since the middle of the past century, when they proved integral to the development of viral vaccines. For decades, cells were studied in essentially two-dimensional environments. The need for three-dimensional ones became apparent, however, with the advent of tissue engineering in the 1990s – 3-D cultures, of course, better replicated the in vivo environment that researchers were seeking to reproduce. With the new dimensionality of cell cultures came the need for a new means of looking at them. Because 3-D cell cultures are thicker and more...Read full article

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    Published: November 2010
    Glossary
    two-photon excited fluorescence
    Two-photon excited fluorescence (TPEF) is a nonlinear optical method that allows imaging of biological cells and living tissue. The advantage of TPEF in comparison to conventional fluorescence microscopy is that it provides natural confocality and allows sectioning of the sample. Because it typically uses near-infrared excitation light, the penetration depth is significantly increased. TPEF is implemented as fast imaging microscopy for noninvasive optical pathology. TPEF has been used in...
    3-D cell cultures3-D tissue constructsBiophotonicsBoasBoppartcell culturesconfocal microscopyengineered skinengineered tissueFeaturesGaryGary BoasGeorgakoudiIreneIrene GeorgakoudiMicroscopymultiphoton microscopyoptical coherence elastographyoptical coherence microscopyPLOS ONEsecond harmonic generationSHGStephenStephen A. Boppartthree-dimensional cell culturestissue engineeringTPEFTuftsTufts Universitytwo-photon excited fluorescenceUniversity of IllinoisUrbana-Champaign

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