Search
Menu
Hamamatsu Corp. - Earth Innovations LB 2/24

Multimodal Imaging: Combining Techniques to Give Microscopists a Boost

Facebook X LinkedIn Email
By Karen Newman, Group Publisher

On the way to conjoining two well-established but disparate imaging modalities, a FLIM (fluorescence lifetime imaging) and CARS microscope was achieved along with the development of an adapter that could bring real change to the way microscopists use their instruments. Working on a grant to study the hepatitis C virus, scientists at National Research Council of Canada, Steacie Institute for Molecular Sciences, in Ottawa, along with colleagues from National Yang-Ming University, Taipei, and Queen’s University, Ontario, sought a modality to study lipids. They decided to combine FLIM...Read full article

Related content from Photonics Media



    Articles


    Products


    Photonics Handbook Articles


    White Papers


    Webinars


    Photonics Dictionary Terms


    Media


    Photonics Buyers' Guide Categories


    Companies
    Published: May 2011
    Glossary
    coherent anti-stokes raman scattering
    A technique whereby two laser beams, one at an excitation wavelength and the second at a wavelength that produces Stokes Raman scattering, interact coherently in a sample, producing a strong scattered beam at the anti-Stokes wavelength.
    fluorescence lifetime imaging
    Fluorescence lifetime imaging (FLIM) is an advanced imaging technique that provides information about the lifetime of fluorescence emissions from fluorophores within a sample. Unlike traditional fluorescence imaging, which relies on the intensity of emitted light, FLIM focuses on the time a fluorophore remains in its excited state before returning to the ground state. This fluorescence lifetime is influenced by the local environment and can be used for various applications in biological and...
    label-free
    Label-free refers to a technique or method that does not require the use of additional labels, tags, or markers to detect or identify specific components or entities. In various scientific and technological applications, labeling often involves attaching fluorescent dyes, radioactive isotopes, or other markers to molecules, cells, or structures of interest. However, in label-free approaches, detection or analysis is performed without the need for these additional labels. Common applications...
    microscope
    An instrument consisting essentially of a tube 160 mm long, with an objective lens at the distant end and an eyepiece at the near end. The objective forms a real aerial image of the object in the focal plane of the eyepiece where it is observed by the eye. The overall magnifying power is equal to the linear magnification of the objective multiplied by the magnifying power of the eyepiece. The eyepiece can be replaced by a film to photograph the primary image, or a positive or negative relay...
    time-correlated single photon counting
    Time-correlated single photon counting (TCSPC) is a technique used in photon counting applications, particularly in the field of experimental physics, biophysics, and fluorescence lifetime imaging microscopy (FLIM). It is a highly sensitive method for measuring the timing of individual photons, providing detailed information about the temporal characteristics of light emission or absorption. Key components and principles of time-correlated single photon counting include: Photon counting: In...
    two-photon fluorescence
    This results from the simultaneous absorption of two photons, each having half the energy needed for excitation and requiring a high spatial and temporal concentration of photons. The ensuing confocal effect confines the excitation to the plane of focus. The technique provides longer observation times for live cell studies.
    Aaron SlepkovAlbert StolowAmericasbiomedicalBiophotonicsCARScoherent anti-Stokes Raman scatteringCommunicationsf-CARSfemtosecond lasersfiber lasersfiber opticsFLIMFLIM-CARSfluorescence lifetime imagingfluorsceinFRETHCVHepatitis C virusImagingIRFKaren Newmanlabel-freemicroscopemicroscopistsMicroscopymultimode fibermultimode imagingNational Research Council of CanadaNational Yang-Ming UniversitynonlinearOlympus Corp.optical microscopyoptical spectroscopyQueen’s UniversityResearch & TechnologySensors & DetectorsspectroscopySteacie Institute for Molecular SciencesTCSPCtime-correlated single photon countingtwo-photon fluorescenceWeb Exclusives

    We use cookies to improve user experience and analyze our website traffic as stated in our Privacy Policy. By using this website, you agree to the use of cookies unless you have disabled them.