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Photoacoustic Microscopy Unlocks Secrets of Optical Absorption

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In vivo, label-free subwavelength-resolution photoacoustic microscopy enables more precise measurement of optical absorption – and, therefore, offers more information.

Chi Zhang, Konstantin Maslov and Lihong V. Wang, Washington University, St. Louis

Mainstream optical microscopy technologies cannot sensitively measure the optical absorption that provides essential physiological information such as the concentration and oxygen saturation of hemoglobin. In our research, we have implemented subwavelength-resolution photoacoustic microscopy (SW-PAM) to provide high-optical-absorption contrast by using a water-immersion optical objective with a 1.23 NA. SW-PAM can detect nonfluorescent endogenous pigments in vivo with 100 percent sensitivity to optical absorption. We found that, by imaging gold nanospheres, SW-PAM approached the...Read full article

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    Published: September 2011
    Glossary
    fluorescence
    Fluorescence is a type of luminescence, which is the emission of light by a substance that has absorbed light or other electromagnetic radiation. Specifically, fluorescence involves the absorption of light at one wavelength and the subsequent re-emission of light at a longer wavelength. The emitted light occurs almost instantaneously and ceases when the excitation light source is removed. Key characteristics of fluorescence include: Excitation and emission wavelengths: Fluorescent materials...
    optical coherence tomography
    Optical coherence tomography (OCT) is a non-invasive imaging technique used in medical and scientific fields to capture high-resolution, cross-sectional images of biological tissues. It provides detailed, real-time, and three-dimensional visualization of tissue structures at the micrometer scale. OCT is particularly valuable in ophthalmology, cardiology, dermatology, and various other medical specialties. Here are the key features and components of optical coherence tomography: Principle of...
    AmericasBasic ScienceBiophotonicscellsChi Zhangconfocal microscopyEndra Inc.Featuresfluorescencegold nanosphereshemoglobinImagingKonstantin MaslovLihong V. WangMicrophotoacoustics Inc.MicroscopyNational Institutes of HealthNIHOCToptical absorptionoptical coherence tomographyoptical microscopyorganellesPAMPATPhotoacoustic MicroscopyPhotoacoustic tomographysubwavelength-resolution photoacoustic microscopySW-PAMtwo-photon microscopyWashington University in St. Louis

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