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Live-Cell Imaging Uses All the Best Acronyms

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Gary Boas, Contributing Editor, [email protected]

Researchers are working to improve FRET, TIRF, FLIM, FRAP and other microscopy methods to get the most accurate data possible. This may be an obvious statement, but live-cell microscopy is an important, even integral, tool in biomedical research. In studies of cell biology, neurobiology, pharmacology and more, it has enabled insights that would not have been possible by imaging fixed cells. It has allowed investigators to observe changes in cell structures over time as well as the ways in which they interact with other cells, proteins and so on – and thus helped to address a wide...Read full article

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    Published: December 2012
    Basic ScienceBiophotonicscamerasCCDCNRSElizabeth BaggaleyfastFLIMFeaturesFLIMfluorescence lifetime imaging microscopyForster resonance energy transferFRETGareth WilliamsImagingJulia WeinsteinJulien Roulluminescent metal complexesMarc TramierMicroscopySanford SimonSensors & DetectorsSergi Padilla-ParraThe National Center for Science ResearchThe Rockefeller UniversityThe University of DurhamThe University of RennesThe University of SheffieldTIRFtotal internal reflection microscopy

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