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Challenges to CARS Microscopy: Light at the End of the Tunnel

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Marie Freebody, Contributing Editor, [email protected]

Coherent anti-Stokes Raman scattering (CARS) microscopy historically has struggled to break into a marketplace already crowded with popular imaging techniques such as fluorescence microscopy and with new superresolution techniques such as STORM (stochastic optical reconstruction microscopy) and STED (stimulated emission depletion microscopy). Although fluorescence microscopy may still be the most commonly used biological imaging tool, it is not without its drawbacks: It is severely limited by the artifacts, photobleaching and cytotoxicity caused by the labels it requires. Not that...Read full article

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    Published: January 2014
    Glossary
    coherent anti-stokes raman scattering
    A technique whereby two laser beams, one at an excitation wavelength and the second at a wavelength that produces Stokes Raman scattering, interact coherently in a sample, producing a strong scattered beam at the anti-Stokes wavelength.
    fluorescence microscopy
    Fluorescence microscopy is a specialized optical imaging technique used in biology, chemistry, and materials science to visualize and study specimens that exhibit fluorescence. Fluorescence is the phenomenon where a substance absorbs light at one wavelength and emits light at a longer wavelength. In fluorescence microscopy, fluorescent dyes or proteins are used to label specific structures or molecules within a sample. The basic principles of fluorescence microscopy involve illuminating the...
    hyperspectral imaging
    Hyperspectral imaging is an advanced imaging technique that captures and processes information from across the electromagnetic spectrum. Unlike traditional imaging systems that record only a few spectral bands (such as red, green, and blue in visible light), hyperspectral imaging collects data in numerous contiguous bands, covering a wide range of wavelengths. This extended spectral coverage enables detailed analysis and characterization of materials based on their spectral signatures. Key...
    photobleaching
    Photobleaching is a phenomenon in which the fluorescence of a fluorophore (a fluorescent molecule or dye) is permanently reduced or eliminated upon prolonged exposure to light. This process occurs due to the photochemical destruction or alteration of the fluorophore molecules, rendering them non-fluorescent. Key points about photobleaching: Mechanism: Photobleaching is typically a result of chemical reactions induced by the absorbed photons. The excessive light exposure causes the...
    pulse shaping
    The use of variations in the power supplied to a laser to change the shape of the output pulse. The technique is used in laser welding, for example, to condition a surface by preheating it at a low power or to anneal a surface at low power after the high-power weld is completed.
    superresolution
    Superresolution refers to the enhancement or improvement of the spatial resolution beyond the conventional limits imposed by the diffraction of light. In the context of imaging, it is a set of techniques and algorithms that aim to achieve higher resolution images than what is traditionally possible using standard imaging systems. In conventional optical microscopy, the resolution is limited by the diffraction of light, a phenomenon described by Ernst Abbe's diffraction limit. This limit sets a...
    Biophotonicsbroadband pulsesCardiff UniversityCARS microscopyChemometricscoherent anti-Stokes Raman scatteringFeaturesfiber lasersfluorescence microscopyfood processinghyperspectral imagingfiberJi-Xin Chenglipidsliving organismsMarie Freebodymyelin sheathpharmaceutical researchphotobleachingpicosecond pulsespulse shapingsecond harmonic generationSRSSTEDStimulated Raman scatteringSTORMSunney Xiesuperresolutionthird harmonic generationtissue engineeringImaging & Sensingcytotoxitylarge scale neuronal imagingThuc LeDesert Research Institutevibrational imagingtwo-photon fluorescence imagingOfferhausHerman OfferhausTwente UniversityStimulated Raman gainstimulated Raman losschirped femtosecond pulsesDaniel CôtéVibrational photo-acousticVPAEndoscopic CARS microscopyspectral decompositionPaola Borrineurodegeneretive applicationsProgress in Lipid Research

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