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Enhanced CARS Enables High-Speed Imaging of Tissues

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An enhanced spectroscopy technique for analyzing biological cells and tissues delivers stronger signals than those attained through conventional practices. A team from the National Institute of Standards and Technology, in conjunction with the Cleveland Clinic, has demonstrated the improved method based on characteristic molecular vibration signatures. A false-color BCARS image of mouse liver tissue picks out cell nuclei in blue, collagen in orange and proteins in green. The image shows an area about 200 µm across. Images Courtesy of Dr. Charles Camp Jr./NIST. The...Read full article

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    Published: July 2014
    Glossary
    raman spectroscopy
    Raman spectroscopy is a technique used in analytical chemistry and physics to study vibrational, rotational, and other low-frequency modes in a system. Named after the Indian physicist Sir C.V. Raman who discovered the phenomenon in 1928, Raman spectroscopy provides information about molecular vibrations by measuring the inelastic scattering of monochromatic light. Here is a breakdown of the process: Incident light: A monochromatic (single wavelength) light, usually from a laser, is...
    AmericasBiophotonicsCleveland ClinicfingerprintImagingLasersLight SourcesMarcus CiceroneMarylandNational Institute of Standards and TechnologyNISTOhioRaman scatteringRaman spectroscopyResearch & Technologyspectroscopybroadband coherent anti-Stokes Raman scatteringBCARSCharles Camp Jr.BioScan

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