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Peering into the Nanoworld with Optical Microscopy

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Marie Freebody, Contributing Editor

From brain mapping to breaking the laws of physics, optical microscopy has made huge leaps forward in recent years. But as new dimensions open up, it’s clear the real work has only just begun. Last year yielded a bumper crop of Nobel Prizes for microscopy innovations. Thanks to advances in optical components and the labeling specificity of fluorescent probes, scientists now have a significantly more powerful tool, offering glimpses deeper than ever into the molecules that make life tick. It was long believed that optical microscopy would never obtain resolution better than...Read full article

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    Published: March 2015
    Glossary
    superresolution
    Superresolution refers to the enhancement or improvement of the spatial resolution beyond the conventional limits imposed by the diffraction of light. In the context of imaging, it is a set of techniques and algorithms that aim to achieve higher resolution images than what is traditionally possible using standard imaging systems. In conventional optical microscopy, the resolution is limited by the diffraction of light, a phenomenon described by Ernst Abbe's diffraction limit. This limit sets a...
    FeaturessuperresolutionMicroscopyOsamooptical microscopyfluorescent probesEric BetzigStefan HellWilliam Esco MoernerMatthew LewOsamo ShimomuraMartin ChalfieRoger TsienOlympusRolf BorlinghausChristoph GrebZeiss MicroscopyDr. Klaus WeisshartSilvio RizzoliKoichi WakataEdvard MoserWesley LegantDr. Johannes AmonMarie Freebody

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