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PI Physik Instrumente - Revolution In Photonics Align ROSLB 3/24

Focus-Tunable Lenses Enable 3-D Microscopy

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David Leuenberger, Optotune AG, and Fabian F. Voigt, University of Zurich

Focus-tunable lenses speed up and enhance three-dimensional imaging capabilities in a diverse set of modern microscopy methods including confocal, two-photon and light-sheet microscopy. Microscopy beginners may be perplexed when they notice that parts of a sample that appear only marginally out of focus often look much more blurred in an image. The depth of field as seen by the human eye seems to be considerably larger than the depth of field seen by a camera. This bewildering effect occurs because the eye accommodates: While looking through a microscope, the user constantly – and...Read full article

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    Published: April 2015
    camerasFeaturesBiophotonicsMicroscopyImagingEuropefocus-tunable lenses3-D imagingtunable lenseselectrically tunable lensesETLin vivo imagingremote focusingshape-changing lensoffset lensETL/OL combinationconfocal microscopytwo-photon microscopy3-D light sheet microscopyDavid LeuenbergerUniversity of ZurichFabian VoigtOptotuneSwitzerland

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