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Fluorescence Lifetime Imaging – Application Simplified…

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Gerhard Holst

For many years the phenomenon of photoluminescence has been used for a variety of purposes in life science applications, ranging from bio markers to sensing actions. Each of the luminophores that are applied has multiple characteristic parameters, which can be exploited for investigations. The most prominent parameter is the luminescent emission itself, the fluorescence or phosphorescence intensity. The intensity is used qualitatively and quantitatively, but the latter strongly depends on the light field and the optical conditions around the luminophore. Similarly it is known that the...Read full article

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    Glossary
    fluorescence lifetime imaging
    Fluorescence lifetime imaging (FLIM) is an advanced imaging technique that provides information about the lifetime of fluorescence emissions from fluorophores within a sample. Unlike traditional fluorescence imaging, which relies on the intensity of emitted light, FLIM focuses on the time a fluorophore remains in its excited state before returning to the ground state. This fluorescence lifetime is influenced by the local environment and can be used for various applications in biological and...
    photoluminescence
    The state of optically excited luminescence. Luminescence refers to the light emitted by excited atoms or ions as they decay to lower energy levels.
    camerasCMOSPCOfluorescence lifetime imagingphotoluminescencelife scienceImagingcamera systemsFLIMAmericas

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