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Viral Method Aids Two-Photon Imaging of Primate Brains

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Tailored viruses that make brain cells manufacture fluorophores could make two-photon microscopy techniques viable for studying primate brains. Tested in marmosets, the technique could, for the first time, allow scientists to study neural activity related to the complicated cognitive and social behaviors of the order of mammals that includes humans. Representative images of GCaMP6f fluorescent signals. Neurons indicated by yellow arrowheads were assumed to be the same between post-injection day 10 and post-injection day 118. Image depth was 200 μm from the cortical surface....Read full article

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    Published: November 2015
    Glossary
    fluorescence
    Fluorescence is a type of luminescence, which is the emission of light by a substance that has absorbed light or other electromagnetic radiation. Specifically, fluorescence involves the absorption of light at one wavelength and the subsequent re-emission of light at a longer wavelength. The emitted light occurs almost instantaneously and ceases when the excitation light source is removed. Key characteristics of fluorescence include: Excitation and emission wavelengths: Fluorescent materials...
    Research & TechnologyAsia-PacificJapanRIKENOsamu SadakaneTetsuo YamamoriImagingMicroscopyBiophotonicsbrainfluorescenceBioScan

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