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3-D AFM Could Advance Understanding of Proteins

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An advanced 3-D atomic force microscope will allow the study of membrane proteins in conditions similar to those found in the body, an improvement that could lead to increased understanding of proteins on the microscopic level and faster drug therapies.

The study of complex proteins that allow information and molecules to pass into and out of a cell has long been restricted by the limitations of one-dimensional force microscopes. Preparation requirements mean that a specimen could not be studied as it would behave in its normal environment.


A 3-D atomic force microscope developed by researchers at the University of Missouri. Images courtesy University of Missouri’s Department of Physics and Astronomy and Department of Biochemistry. 


Researchers at the University of Missouri (MU), using a traditional one-dimensional force microscope as a guide, have added another laser to measure the second and third dimensions of tip movement. This provided real-time access to the measurement of peaks and valleys in the membrane protein and dynamic changes in those structures.

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Researchers scattered a focused laser directly off an atomic force microscope tip apex to rapidly and precisely measure the tapping tip trajectory in a 3-D space.


“Using this new laser, we collect the back-scattered light from not only the cantilever holding the needle, but also the tip of the needle that gives additional measurements,” King said. “This added flexibility allows us to collect information faster and allows our microscope to work in near-native conditions in fluid like those found in the cell, yielding more realistic results.”

By studying how the shape of proteins change, researchers can determine how drugs bind and interact with cells, said Gavin King, assistant professor of physics and astronomy in the College of Arts & Science at MU, and joint assistant professor of biochemistry. The membrane protein information can assist pharmaceutical companies in determining which molecules to pursue.

The work was published in Nano Letters.

For more information, visit www.missouri.edu.

Published: January 2014
Glossary
astronomy
The scientific observation of celestial radiation that has reached the vicinity of Earth, and the interpretation of these observations to determine the characteristics of the extraterrestrial bodies and phenomena that have emitted the radiation.
atomic force microscope
An atomic force microscope (AFM) is a high-resolution imaging and measurement instrument used in nanotechnology, materials science, and biology. It is a type of scanning probe microscope that operates by scanning a sharp tip (usually a few nanometers in diameter) over the surface of a sample at a very close distance. The tip interacts with the sample's surface forces, providing detailed information about the sample's topography and properties at the nanoscale. Key features and principles of...
nano
An SI prefix meaning one billionth (10-9). Nano can also be used to indicate the study of atoms, molecules and other structures and particles on the nanometer scale. Nano-optics (also referred to as nanophotonics), for example, is the study of how light and light-matter interactions behave on the nanometer scale. See nanophotonics.
3-DAFMAmericasastronomyatomic force microscopebiochemistryBiophotonicsBioScanImagingmembrane proteinMicroscopyMissourinanoNano LettersphysicsResearch & TechnologyUniversity of MissouriGavin King

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