BioPhotonics Preview - September/October 2022

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Here is your first look at the editorial content for the upcoming September/October issue of BioPhotonics.


Multiphoton Microscopy

Multiphoton imaging is vastly becoming a common analytical tool for 3D time-lapse, deep, and high-resolution imaging of live animals and animal models. Key design drawbacks in instrumentation limit traditional devices from being accessible and easy-to-use. Nonlinear techniques have distinct advantages over traditional confocal methods due to high spatial resolution, superior optical sectioning, and deeper penetration with low photodamage and phototoxicity. Additionally, a very broad variety of label-free, label-dependent and indigenous signatures exist that can be probed. Prospective Instruments brings laser scanning multiphoton microscopy out of the laboratory and makes it available to everyone. A modular design, completely integrated with a broadly tunable fiber laser, allows the user to explore a wide variety of applications in any indoor environment. In this article we describe the new philosophy combining a portable, turnkey platform and multimodality. We present results from imaging zebrafish and Drosophila brain samples to demonstrate the unique benefits of multiphoton imaging and its application in the fields of biomedical research. Observing in detail yields deeper insights into human physiology and pathology.

Key Technologies: multiphoton microscopy, lasers

Laser Scanning & Diagnostics

Scan head control systems are already used in many industrial laser-based applications requiring the highest precision and throughput. Now the benefits of scanning with zero tracking error are conquering ophthalmic applications where scan pattern fidelity is critical for the outcome of the patient. The smart control technology enables different and new strategies compared to previous controls which are affected by tracking error, especially when using circles, spirals and free form structures. This results in new opportunities as well as successful and reproducible refractive surgeries. By using the full dynamic capabilities of the galvo motor, throughput can be increased which means that operation times are reduced.

Key Technologies: lasers


Optogenetics is a novel neuroscience tool that facilitates studies of brain function by selectively activating photo-sensitive neurons and measuring the induced brain activity. However, brain tissue heavily affects light illumination since scattering and absorption events occur deep into the brain. Computer Generated Holograms are employed for neural photostimulation as they provide arbitrary illumination patterns, but they still suffer from these events. Our approach resolves this limitation by engaging advanced optimization algorithms in the hologram design procedure. In particular, brain tissue is simulated as a series of parallel phase masks. Each phase mask corresponds to a different depth into the tissue and implies a shift onto the propagated light field based on the tissue properties. Our advanced algorithms take into account scattering effects of brain tissue in the hologram optimization process. As such, the specificity of holographic optogenetic stimulation is enhanced fostering arbitrary neural illumination.

Key Technologies: Optogenetics, holography

OCT System Design

Expanding the clinical application of OCT imaging will require new features and form factors to address the varied needs of healthcare providers. The dramatic arrival of optical coherence tomography to ophthalmology in the early 2000s is the sort of “ scientific legend” that invigorates researchers and entrepreneurs to this day. This novel imaging technique, first studied in vivo human retina by Swanson et al. (1993), and commercialized by 2005, provides a window into the delicate layers of the retina at the resolution required to monitor sight-threatening diseases. Lumedica's OQ EyeScope will make this technology accessible to busy clinics and underdeveloped healthcare systems by providing a compact, portable and affordable, OCT screening device. Discussion with other industry leaders will lay out new paths for OCT including home use for retinal screening, OCT-enhanced ear exams, handheld probes for pediatric use, and others. According to Ryan Shelton at PhotoniCare Inc. OCT-enhanced ear exams remove the guesswork for physicians. Dermatological applications are on the horizon with Dual-axis OCT demonstrating improved imaging depth and resolution.

Key Technologies: OCT

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Published: June 2022

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