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Breakthroughs in Live Imaging

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Fluorescence lifetime imaging microscopy (FLIM) allows researchers to learn about molecular-level interactions in live specimens. The process yields more robust results than intensity-based imaging techniques, and it’s been part of researchers’ toolboxes for around a decade. It also holds great promise for surgeons and other clinicians because it can distinguish nondestructively between diseased and normal tissue. But FLIM also is expensive and difficult to use.

Marcia StamellWhen we asked a panel of researchers and industry experts to comment on this powerful technique, we expected rich and informative answers. And we certainly weren’t disappointed. We received so much insightful material that we couldn’t print it all in the magazine’s pages. “FLIM Delivers Intracellular Images Based on Differences,” (read article), with discussions from each of the seven experts who wrote us. We urge you to go to, where the story continues with additional responses from each of our panelists.

Also in the magazine:

• Russell Ulbrich of Olympus America Inc. discusses another microscopy technique of imaging live samples at the molecular level. “Challenges and Opportunities in Superresolution” surveys a variety of superresolution methods, their capacities and the limitations they present (read article).

• Richard Meester and Sander de Jonge at Quest Medical Imaging describe innovative uses of spectral-based real-time imaging in the operating room. Their highly informative article, “Fluorescence Imaging Enters the Surgical Suite” (read article).

• Contributing Editor Hank Hogan writes about applications for medical lasers beyond ablation and tissue removal in “Medical Lasers Cut and Heal” (read article).

• Jon Holmes of Michelson Diagnostics outlines a route to wider clinical adoption of advanced technology in this month’s Biopinion. Don’t miss “Adoption Depends on Meeting Clinical Needs” (read article).

Enjoy the issue.

May/Jun 2017
EditorialMarcia Stamell

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