Fluorescence Microscopy: Getting the Picture

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fluorescence microscopy

Observation of samples using excitation produced fluorescence. A sample is placed within the excitation laser and the plane of observation is scanned. Emitted photons from the sample are filtered by a long pass dichroic optic and are detected and recorded for digital image reproduction.

fluorochrome

The combination of the organic dye in a stained specimen and the antibodies produced that is detected by exposure to light.

c-mount

A standard lens interface initially made for 16mm movie cameras and now used primarily on closed-circuit television cameras. It is a 1-in.-diameter, 32-thread-per-inch interface with a flange-to-image plane distance of 0.69 in.

dark current

The current that flows in a photodetector when there is no optical radiation incident on the detector and operating voltages are applied.