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Fluorescence Microscopy: Getting the Picture

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Published: August 2019
Glossary
fluorescence microscopy
Fluorescence microscopy is a specialized optical imaging technique used in biology, chemistry, and materials science to visualize and study specimens that exhibit fluorescence. Fluorescence is the phenomenon where a substance absorbs light at one wavelength and emits light at a longer wavelength. In fluorescence microscopy, fluorescent dyes or proteins are used to label specific structures or molecules within a sample. The basic principles of fluorescence microscopy involve illuminating the...
fluorochrome
The combination of the organic dye in a stained specimen and the antibodies produced that is detected by exposure to light.
c-mount
A standard lens interface initially made for 16mm movie cameras and now used primarily on closed-circuit television cameras. It is a 1-in.-diameter, 32-thread-per-inch interface with a flange-to-image plane distance of 0.69 in.
dark current
The current that flows in a photodetector when there is no optical radiation incident on the detector and operating voltages are applied.
quantum efficiency
Quantum efficiency (QE) is a measure of the effectiveness with which a device or system, typically in the context of photonics or electronics, converts incoming photons (light) into a useful output signal or response. It is expressed as a ratio or percentage and quantifies the number of electrons or charge carriers generated in response to the incident photons. In other words, quantum efficiency provides a measure of how well a device can capture and utilize photons to produce an electric...
gige
GigE, short for gigabit Ethernet, refers to a standard for high-speed Ethernet communication, capable of transmitting data at rates of up to 1 gigabit per second (Gbps), or 1000 megabits per second (Mbps). GigE is an extension of the ethernet family of networking technologies, which is widely used for local area network (LAN) communication in homes, businesses, and data centers. Key features and characteristics of gigabit Ethernet include: Speed: GigE offers significantly higher data...
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