Search Menu
Photonics Media Photonics Marketplace Photonics Spectra BioPhotonics Vision Spectra Photonics Showcase Photonics ProdSpec Photonics Handbook

GFPs Measure Intracellular Heat

Facebook Twitter LinkedIn Email
BARCELONA, Spain, May 29, 2012 — A noninvasive method that measures internal cell temperatures using green fluorescent proteins (GFPs) could help scientists to learn more about cellular processes and to distinguish healthy cells from cancerous ones.

The technique, which was developed by a European research team led by the Institute of Photonic Sciences (ICFO), measures intracellular heat without altering the cells’ metabolism, and more quickly and precisely than earlier methods.

“A unique characteristic of our method is that it does not alter any cellular process,” said Romain Quidant, an ICFO researcher and coordinator of the study.

The green fluorescent proteins help measure intracellular heat. (Image: Richard Wheeler)

Unlike other techniques, this method does not stress or alter the behavior of the cell because it does not need to be inserted into any molecules or any other synthetic nano-object that is sensitive to the internal temperature.

Intracellular temperatures make it possible to study the body’s energy flow during the uncontrolled spreading of cancer cells. Measurement of energy transmission can help investigate processes such as gene expression, metabolism and cell splitting.

Using fluorescence polarization anisotropy, the team measured the polarization difference between light that fluorescent molecules received and that which they emitted later.

“This difference in polarization (anisotropy) is directly connected to the rotating of the GFP molecules and therefore with temperature,” Quidant said.

Biologists will be able to implement this technique in experimental setups and to obtain the cell temperature as another observable detail, the scientists said.

Harnessing the ability to measure intracellular activity could be instrumental in developing a field that has not been widely studied: thermal biology at the cellular level.

Next, the ICFO team plans to improve the method’s sensitivity and resolution. To achieve this, its members hope to fine-tune the properties of the fluorescent proteins to optimize the detection method of their “thermometer.”

The research was published in Nano Letters.

For more information, visit:
May 2012
The technology of generating and harnessing light and other forms of radiant energy whose quantum unit is the photon. The science includes light emission, transmission, deflection, amplification and detection by optical components and instruments, lasers and other light sources, fiber optics, electro-optical instrumentation, related hardware and electronics, and sophisticated systems. The range of applications of photonics extends from energy generation to detection to communications and...
With respect to light radiation, the restriction of the vibrations of the magnetic or electric field vector to a single plane. In a beam of electromagnetic radiation, the polarization direction is the direction of the electric field vector (with no distinction between positive and negative as the field oscillates back and forth). The polarization vector is always in the plane at right angles to the beam direction. Near some given stationary point in space the polarization direction in the beam...
anisotropybiologyBiophotonicscell splittingcellular processesEuropefluorescent polarization anisotropyFPAgene expressionGFPsgreen fluorescent proteinsICFOInstitute of Photonic Sciencesinternal temperature of cellsintracellular heatintracellular thermometermetabolismmetastasisphotonicspolarizationResearch & TechnologyRomain QuidantSpainTest & Measurement

back to top
Facebook Twitter Instagram LinkedIn YouTube RSS
©2023 Photonics Media, 100 West St., Pittsfield, MA, 01201 USA, [email protected]

Photonics Media, Laurin Publishing
x We deliver – right to your inbox. Subscribe FREE to our newsletters.
We use cookies to improve user experience and analyze our website traffic as stated in our Privacy Policy. By using this website, you agree to the use of cookies unless you have disabled them.