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Kinetic River Develops Autofluorescence Removal Technology

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Flow cytometry instrumentation company Kinetic River Corp. completed a small business innovation research (SBIR) project funded by the National Institutes of Health (NIH). The award funded the development of the company’s “Colorado” automated autofluorescence removal technology.

“Through a combination of hardware and algorithm development, we have been able to demonstrate the removal of the autofluorescence contribution from a population of exogenously stained eosinophils — a cell type with notoriously high autofluorescence signal,” said Giacomo Vacca, president of Kinetic River.

The completion of the Phase I program paves the way for further development and commercialization of a four-laser, 16-channel Colorado instrument with fully automated removal of the contribution from cellular autofluorescence, the goal of a Phase II SBIR proposal recently submitted to the NIH.

BioPhotonics
May/Jun 2021
GLOSSARY
flow cytometry
A method of measuring the characteristics of microscopic particles, usually cells, as they flow in a fluid stream through a beam of light. Particles may be stained with fluorescent dye and the fluorescence detected via laser illumination.
fluorescence
The emission of light or other electromagnetic radiation of longer wavelengths by a substance as a result of the absorption of some other radiation of shorter wavelengths, provided the emission continues only as long as the stimulus producing it is maintained. In other words, fluorescence is the luminescence that persists for less than about 10-8 s after excitation.
Businessresearchresearch & developmentcytometryflow cytometryfluorescencelasersAmericasSBIRSBIR awardSBIR contractSBIR grantSBIR Phase ISBIR Phase IIBioScanlight speed

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