Microscopy handbook
With more than 122 contributing authors, the third
edition of the Handbook of Biological Confocal Microscopy is an authoritative
source of information on all forms of 3-D light microscopy and on related techniques
and instrumentation. It has more than 1000 pages, including 53 chapters, three appendices
and numerous color illustrations. It details microscope parts, digital aspects of
data acquisition, properties of fluorescent dyes, the techniques of 3-D specimen
preparation, and the limitations and practical complexities of quantitative confocal
fluorescence imaging. Chapters address laser sources and objective lenses for confocal
microscopy, visualization systems for multidimensional confocal laser scanning microscopy
images, the specimen illumination path and its effect on image quality and intermediate
optics in Nipkow disk microscopes. James Pawley, ed.; Springer, New York, 2006;
$129.
Published: September 2006