Close

Search

Search Menu
Photonics Media Photonics Buyers' Guide Photonics Spectra BioPhotonics EuroPhotonics Vision Spectra Photonics Showcase Photonics ProdSpec Photonics Handbook
More News
Member Exclusive

Novel Multiphoton Imaging Techniques Reveal Brain’s Secrets

Facebook Twitter LinkedIn Email Comments
Two-photon microscopy has surpassed confocal imaging for the study of intact, living tissue. Three-photon imaging is not far behind and is already allowing researchers to image at depths.

FAROOQ AHMED, CONTRIBUTING EDITOR

Nearly two decades ago, Winfried Denk and Karel Svoboda argued in the journal Neuron that “multiphoton imaging is more than a gimmick”1. At the time, confocal microscopy — combined with advancements in fluorescent reporter systems and an increase in desktop computing power — had set a high standard for 3D imaging of biological specimens. But Denk, a German physicist at Bell Laboratories in Murray Hill, N.J., who developed two-photon imaging in 1990 when he was a

Member Exclusive: To read the complete article, please Login or Register

Photonics Handbook
GLOSSARY
excitation
1. The process by which an atom acquires energy sufficient to raise it to a quantum state higher than its ground state. 2. More specifically with respect to lasers, the process by which the material in the laser cavity is stimulated by light or other means, so that atoms are converted to a semistable state, initiating the lasing process.
photobleaching
A process that helps optical fibers recover from damage induced by radiation. When silica is irradiated, bonds break and attenuation increases. Light in the fiber assists in recombining the species released by the broken bonds, decreasing attenuation.
MicroscopyExcitationphotobleachingphotodamagespatial filterstwo-photon microscopymultiphoton imagingconfocal imagingopticsFeaturesBiophotonics

Comments
back to top
Facebook Twitter Instagram LinkedIn YouTube RSS
©2019 Photonics Media, 100 West St., Pittsfield, MA, 01201 USA, info@photonics.com

Photonics Media, Laurin Publishing
x We deliver – right to your inbox. Subscribe FREE to our newsletters.
We use cookies to improve user experience and analyze our website traffic as stated in our Privacy Policy. By using this website, you agree to the use of cookies unless you have disabled them.