New developments in imaging technology, in combination with advanced microscopy techniques, are enabling new applications for fluorescence sensing.JASPER BOSCH, LAMBERT INSTRUMENTS BVDetecting fluorescence light via imaging and fluorescence sensing can take researchers and clinicians into the body, allowing them to visualize samples and biological progression of areas such as cancer detection or cardiovascular function. The fluorescence light contains the information about the sample that a fluorophore has detected, so recording it with great precision is important for the accuracy of the measurement. In recent years, there have been many improvements in the fluorophores and imaging technology involved in fluorescence sensing. 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