New developments in imaging technology, in combination with advanced microscopy techniques, are enabling new applications for fluorescence sensing.JASPER BOSCH, LAMBERT INSTRUMENTS BVDetecting fluorescence light via imaging and fluorescence sensing can take researchers and clinicians into the body, allowing them to visualize samples and biological progression of areas such as cancer detection or cardiovascular function. The fluorescence light contains the information about the sample that a fluorophore has detected, so recording it with great precision is important for the accuracy of the measurement. In recent years, there have been many improvements in the fluorophores and imaging technology involved in fluorescence sensing. These developments have improved the...Read full articleRelated content from Photonics MediaARTICLESNovel Multiphoton Imaging Techniques Reveal Brain’s SecretsNearly two decades ago, Winfried Denk and Karel Svoboda argued in the journal Neuron that “multiphoton imaging is more than a gimmick”1. At the time, confocal microscopy — combined...BioPhotonics Feb/Mar 2019 IssueFluorescence Microscopy: Getting the Picture RightFluorescence microscopy is a very broadly used term covering numerous applications. It ranges from basic life science applications such as time-lapse cell viability assays — in which dead cells...BioPhotonics September 2019 IssueAO-OCT Comes into FocusAdaptive optics (AO) technology is often used to correct for wavefront distortions imparted when light travels through complex optical systems, enhancing image resolution and facilitating...BioPhotonics Feb/Mar 2019 IssuePortable Microscopy Leverages Advancements in Electronics and ComputingSpurred by Moore’s law, the principle that the number of transistors on a microchip doubles every two years, the miniaturization of electronic components has resulted in optical devices...BioPhotonics Sep/Oct 2020 IssuePRODUCTSBack Illuminated sCMOS by PCOUnique technology comes from evolution, combining existing and new technology. When PCO’s tried and trusted sCMOS cameras pool forces with modern back illuminated (bi) sensor technology,...Promoted Content January 2019 IssueBack Illuminated sCMOS by PCOUnique technology comes from evolution, combining existing and new technology. When PCO’s tried and trusted sCMOS cameras pool forces with modern back illuminated (bi) sensor technology,...Promoted Content December 2018 Issuepco.edge 4.2 bi: Back Illuminated sCMOSInnovations aren’t always about having that one big new idea. Unique technology also comes from evolution, combining existing and new technology. When PCO’s tried and trusted pco.edge...Promoted Content November 2018 Issuepco.flim: Frequency Domain FLIM CameraThe pco.flim is the first Frequency Domain FLIM camera using a two tap CMOS sensor. Synchronized modulation of the light detection of the pixels and the fluorescence excitation light enables the...Promoted Content December 2019 IssuePHOTONICS HANDBOOK ARTICLESFluorescence Lifetime Imaging: Choosing the Best Approach: Fluorescence lifetime imaging microscopy (FLIM), which measures the spatial distribution of the fluorescence decay, can be achieved either in the time or in the frequency domain. Each approach exhibits advantages and disadvantages, depending on the type of light detector and acquisition method employed.The term fluorescence is often applied as a synonym for photoluminescence, although luminescence actually covers fluorescence and phosphorescence. Both of these terms describe the process of...Photonics HandbookAspheric Lenses: Design Considerations: This first of two articles will cover design and manufacturing options for aspheres; the second will include more detail on manufacturing and testing processes. These articles will provide the tools optics designers need to tailor their designs to the appropriate manufacturing process and to optimize the cost of component fabrication.Aspheric surfaces are powerful tools that combine the optical corrections of multiple lenses into a single element (Figure 1) and affect performance in ways that spherical optics cannot. For example,...Photonics HandbookSuperresolution Microscopy: An Imaging Revolution: Despite tremendous accomplishments in recent years, superresolution microscopy is a relatively young field, and confusion exists in this domain of many abbreviations. However, this makes for captivating times as superresolution microscopy opens the door to wider imaging applications.Superresolution optical microscopy, for which the Nobel Prize was awarded to Eric Betzig, Stefan Hell, and William Moerner in 2014, has been one of the most momentous developments in the life...Photonics HandbookOptical System Optimization: Analyzing the Effects of Stray Light: Stray light can impede the performance of any optical system. With a deeper understanding of stray light, and the right tools, optical engineers can predict and compensate for its effects in order to improve quality.Electrical engineers are very familiar with the effects of shot noise, thermal noise, flicker noise and crosstalk, and recognize how these effects can reduce the signal-to-noise ratio (SNR) in their...Photonics HandbookWHITEPAPERSSeeing Into the Future with 3D Machine Vision3D machine vision was developed to enable automated quality control, but it is now being applied much more widely, in concert with automation and machine learning. Stereo vision, Time-of-Flight (ToF)...Photonics.com 9/1/2019Fast Volumetric and Off-Angle Brain Imaging with the FVMPE-RS® Multiphoton MicroscopeMultiphoton microscopy has become the primary tool for in vivo studies of brain structure and activity at the cellular level in animal models ranging from small mice to larger mammals such as...Photonics.com 5/1/2019Are Large Image Sensors A Perfect Fit for Large Field of View Microscope Applications?In the last years nearly all microscope manufacturers used image circles with 18 mm diameter to image their field of view to cameras connected to their microscopes. Only recently some microscope...Photonics.com 12/2/2019Is It True That Cooled Cameras are More Sensitive than Non-cooled Cameras?The temperature has a significant impact on certain noise sources prevalent in cameras, which contribute to the total readout noise of an image sensor. When electronic devices are operated...Photonics.com 3/19/2019WEBINARSFiber Optic Sensors: Fundamentals and ApplicationsFiber optic sensing technology has evolved over 60 years of development and commercialization. While many applications are for point sensors, the most rapid expansion has been for distributed fiber...Photonics.com 9/24/2015An Introduction to Back Illuminated sCMOS CamerasScientific CMOS (sCMOS) cameras are increasingly becoming detectors of choice for a range of quantitative imaging and spectroscopy applications, from astronomy to biological sciences. This webinar,...Photonics.com 2/14/2017Imaging Applications in Quantum ResearchQuantum technology, an exciting and evolving field combining physics and engineering, applies the properties of quantum mechanics to next-generation computing applications. It has the potential to...Photonics.com 9/26/2018State-of-the-Art Ultrasensitive Imaging Technologies for Microscopy - Choosing the Best SolutionThe recent introduction of true scientific CMOS (sCMOS) technology has caused considerable speculation as to how interline CCD, Electron Multiplying CCD and sCMOS detection technologies compare. This...Photonics.com 12/1/2011PHOTONICS DICTIONARY TERMSphotoconductive antennaPhotoconductive antenna (PCA) is a semiconductor element that generates or detects high-frequency electromagnetic signals. Compact PCAs based on gallium arsenide (GaAs) or indium gallium arsenide...EDU.photonics.com 3/5/2018coded aperture imagingAn imaging process in which the single opening of a simple pinhole camera is replaced with many openings called, collectively, the aperture. The recorded picture, which consists of many overlapping...EDU.photonics.com 9/8/2009noise equivalent delta temperature (NEDT)In a thermal imaging system, the change in temperature that yields a signal-to-noise ratio of unity.EDU.photonics.com 9/8/2009Volumetric ImagingA technique to capture a 3D volume of activity in a single 2D image. This contrasts with conventional imaging techniques that produce two-dimensional replications from one viewing point.EDU.photonics.com 3/5/2018MEDIASupporting the Photonics Industry from the Laboratory - LIGHT MATTERS 01.2018On this month's episode of Light Matters, we take you to central Massachusetts as Worcester Polytechnic Institute and Quinsigamond Community College take on a comprehensive joint photonics laboratory...Photonics.com 1/18/2018Light Matters Extra: Taking Photonics Innovations from the Lab to the Operating Room - 01.2018In this month's Light Matters Extra, we give you an in-depth look at the relationship between researchers and scientists at the University of California Davis, and surgeons and clinicians at nearby...Photonics.com 1/18/2018Sneak Preview: Laser World of Photonics 2017: Bridging Research & IndustryLaser World of Photonics — sponsored by Messe München International — provides a spotlight for promising newcomers, industry veterans and innovative companies. And with tens of thousands of people...Photonics.com 6/12/2017PCO-Tech Inc.PCO-Tech Inc. introduces their newest line of sCMOS cameras - the pco.panda 4.2 and the pco.panda 4.2 back illuminated (bi) camera. Both cameras provide high quantum efficiency with low dark current...Photonics.com 3/21/2018PHOTONICS BUYERS' GUIDE CATEGORIESScientific CMOS Cameras (sCMOS)sCMOS Image Sensors3D ScannersStereolithography ServicesCOMPANIESPCO-TECH Inc.PCO – three decades as Pioneers in Cameras and Optoelectronics PCO is a Pioneer in Cameras and Optoelectronics with more than 30 years of expert knowledge and experience developing and...Photonics Buyers' GuideBasler AGBasler AG – We get the picture Long-lasting digital cameras with highest reliability and outstanding image quality Basler is the leading manufacturer of high-quality digital cameras...Photonics Buyers' GuideDiffraction LimitedFor more than 25 years, Diffraction Limited has been the global leader in Astronomy and Scientific Imaging Solutions — continually delivering unsurpassed products, software and services to a...Photonics Buyers' GuideiX Cameras Inc.iX Cameras is a world-leading technology and product company specializing in the field of high-speed (slow motion) imaging. Based on proprietary innovative technologies, we design, build, and sell...Photonics Buyers' Guide