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Bioapplications of Combined Optical and AFM Imaging Techniques

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Nicholas A. Geisse, Asylum Research

The atomic force microscope (AFM) has seen exciting and promising growth in its combination with optical microscopy. Although new optical techniques developed in the past few years have begun to push traditional limits, the lateral and axial resolutions of optical microscopes typically are limited by the optical elements in the microscope as well as by the diffraction limit of light. One of the most powerful abilities of optical microscopy, however, is its capacity to image through the entire depth of certain samples with chemical specificity using a plethora of label-conjugated markers...Read full article

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