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Hamamatsu Corp. - Earth Innovations LB 2/24

CCD camera discriminates among fluorophores with nanosecond lifetimes

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David Shenkenberg

Fluorescence lifetime imaging microscopy (FLIM) can make quantitative measurements when other techniques cannot. For example, FLIM can discriminate among multiple fluorophores, even in turbid backgrounds such as tissues and cells. However, detecting these subtle differences requires imaging equipment with high temporal resolution. A new high-resolution time-gated CCD camera can distinguish among fluorophores with nanosecond lifetimes, and it can separate fluorophores from a confounding turbid fluorescent medium. In the past, researchers have recorded lifetime images using image-intensified...Read full article

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    Published: March 2007
    BiophotonicsMicroscopyNews & Features

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