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3D Visualization Method Reveals Molecular Position, Orientation, Oscillations

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Researchers at the University of Rochester and the Fresnel Institute in France developed a method for visualizing molecules’ position and orientation in 3D, as well as their oscillations. The technique could allow for greater insights into the biological processes involved when a cell and the proteins that regulate its functions react to a COVID-19 virus. The technology, coordinate and height superresolution imaging with dithering and orientation (CHIDO), designed and built by co-lead authors Valentina Curcio, a Ph.D. student in Sophie Brasselet’s group at the Fresnel...Read full article

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    Published: October 2020
    Glossary
    fluorescence microscopy
    Fluorescence microscopy is a specialized optical imaging technique used in biology, chemistry, and materials science to visualize and study specimens that exhibit fluorescence. Fluorescence is the phenomenon where a substance absorbs light at one wavelength and emits light at a longer wavelength. In fluorescence microscopy, fluorescent dyes or proteins are used to label specific structures or molecules within a sample. The basic principles of fluorescence microscopy involve illuminating the...
    polarization
    Polarization refers to the orientation of oscillations in a transverse wave, such as light waves, radio waves, or other electromagnetic waves. In simpler terms, it describes the direction in which the electric field vector of a wave vibrates. Understanding polarization is important in various fields, including optics, telecommunications, and physics. Key points about polarization: Transverse waves: Polarization is a concept associated with transverse waves, where the oscillations occur...
    birefringence
    Birefringence is an optical property of certain materials that causes them to exhibit different refractive indices for light of different polarizations. In other words, when light passes through a birefringent material, it splits into two separate rays, each traveling with a different speed and direction. This phenomenon is also known as double refraction. The difference in refractive indices for the two polarizations is a characteristic feature of birefringent materials. The separation of...
    Research & TechnologyOpticsRochesterUniversity of RochesterUniversity of Rochester’s Institute of OpticsMicroscopyfluorescence microscopyabbe diffraction limitAbbe limit3D imagingpolarizationpolarization analysisoscillateconfocalconfocal microscopebirefringencebirefringentsingle moleculeAmericasEuropecollaborationTech Pulse

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