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Correction Systems for Deep Imaging Drive More Precise Views

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Imaging via multiphoton microscopy is challenging at deeper levels, especially in the study of delicate structures. Automated spherical aberration correction systems are addressing the problem.

CARLO ALONZO, OLYMPUS CORP. OF THE AMERICAS

Multiphoton microscopy is widely regarded as the primary technique for deep imaging in live tissue. However, image brightness and resolution are typically poorer at deep sites because of increased optical spherical aberration compared to superficial locations. This makes it difficult to study fine structures, such as dendritic spines in deep brain regions. Microscope objectives equipped with adjustable correction collars can compensate for spherical aberration and improve resolution by moving internal lens elements. Motorized correction collars facilitate more precise and convenient...Read full article

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