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DGC Helps Illuminate Mechanisms of Alcohol Dependency

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Dodt gradient contrast microscopy offers a powerful means of imaging colorless specimens and can be integrated with patch-clamp studies.

JOHN WINGERD, SISKIYOU CORP.

Imaging transparent, colorless specimens has always presented a challenge in optical microscopy. In many applications, it is not desirable or even possible to use fluorescent dyes, stains or transgenic probes to render target objects more visible. While differential image contrast (DIC), often in the form of the Nomarski microscope, is widely employed, its optical complexity can cause limitations when the contrast image is combined with some other measurement modality or imaging technique (such as patch-clamp measurements or optogenetic stimulation, or even simply epi-fluorescence). ...Read full article

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