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Donors and Acceptors: New Developments in FRET

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By Gary Boas, News Editor
[email protected]

Förster resonance energy transfer, or FRET, has contributed to advances in a host of applications. Measuring the energy transfer between donor and acceptor dyes attached to two ends of a molecule, or to two different molecules brought close together, enables researchers to probe a range of biological processes, including protein-protein and protein-DNA interactions, ligand-receptor binding, and protein conformational changes in response to a biological stimulus. It also allows them to explore spatial relationships in biological structures and macromolecules by monitoring the distance between...Read full article

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    Published: September 2009
    Glossary
    bioluminescence
    Heatless light emissions from living organisms caused by the combination of oxygen and pigments such as luciferin.
    biomarker
    A biomarker, short for biological marker, refers to a measurable and quantifiable indicator of a biological condition, process, or response. Biomarkers can be substances or characteristics that are objectively measured and evaluated as an indicator of normal biological processes, pathogenic processes, or pharmacological responses to therapeutic interventions. These markers are often used in medical and scientific contexts to assess health status, diagnose diseases, monitor treatment outcomes,...
    fluorescence
    Fluorescence is a type of luminescence, which is the emission of light by a substance that has absorbed light or other electromagnetic radiation. Specifically, fluorescence involves the absorption of light at one wavelength and the subsequent re-emission of light at a longer wavelength. The emitted light occurs almost instantaneously and ceases when the excitation light source is removed. Key characteristics of fluorescence include: Excitation and emission wavelengths: Fluorescent materials...
    microrna
    MicroRNAs (miRNAs) are a class of small, single-stranded RNA molecules, typically consisting of about 19 to 24 nucleotides, that play important roles in the regulation of gene expression in cells. They are involved in post-transcriptional regulation, meaning they influence the translation of messenger RNA (mRNA) into proteins after the mRNA has been transcribed from DNA. The process by which miRNAs regulate gene expression involves several steps: Transcription: miRNA genes are transcribed...
    photobleaching
    Photobleaching is a phenomenon in which the fluorescence of a fluorophore (a fluorescent molecule or dye) is permanently reduced or eliminated upon prolonged exposure to light. This process occurs due to the photochemical destruction or alteration of the fluorophore molecules, rendering them non-fluorescent. Key points about photobleaching: Mechanism: Photobleaching is typically a result of chemical reactions induced by the absorbed photons. The excessive light exposure causes the...
    photonics
    The technology of generating and harnessing light and other forms of radiant energy whose quantum unit is the photon. The science includes light emission, transmission, deflection, amplification and detection by optical components and instruments, lasers and other light sources, fiber optics, electro-optical instrumentation, related hardware and electronics, and sophisticated systems. The range of applications of photonics extends from energy generation to detection to communications and...
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