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Double-Helix Point Spread Function Delivers Precise Extended-Depth Microscopy

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A simple modification allows researchers to extract more data in a single image, reducing the images needed to fully reconstruct a cell.

KATIE HEISER AND LESLIE KIMERLING, DOUBLE HELIX OPTICS LLC

Light microscopy is a powerful method used in many scientific disciplines, including the life sciences, to visualize finer details in samples. The use of fluorescence enables specific targeting and labeling of biomolecules and other chemicals. However, in light microscopy there are trade-offs between the time it takes to acquire an image, the resolution of the resulting image, and photobleaching or damage to the sample. Recent developments in the field have pushed these limitations. A major development is superresolution imaging. This enables researchers to break the diffraction barrier...Read full article

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