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Endospectroscopic Imaging Takes Optical Biopsies to the Next Level

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Raman-based spectroscopy methods promise fast and label-free in vivo diagnosis.

IWAN W. SCHIE AND ALEKSANDAR LUKIC, LEIBNIZ INSTITUTE OF PHOTONIC TECHNOLOGY, AND JÜRGEN POPP, LEIBNIZ INSTITUTE OF PHOTONIC TECHNOLOGY AND INSTITUTE OF PHYSICAL CHEMISTRY, FRIEDRICH-SCHILLER UNIVERSITY

Spectroscopy has the potential to provide pathologists with fast in vivo tissue characterization to determine tumor type and grade and to delineate tumor margins. However, extending the applicability of microspectroscopy to in vivo imaging requires suitable optical fiber probes for an endoscopic inspection of difficult-to-access body regions. The engineering process is challenging. Besides the miniaturization of microscopy, it requires innovative scanning, excitation light delivery and signal collection, as well as robust and alignment-free light sources. Figure 1. Multiphoton image of...Read full article

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