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FLIM Delivers Intracellular Images Based on Differences

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Fluorescence lifetime imaging microscopy derives its information from the decay rates of individual fluorophores. It is opening up understanding of cell metabolism, inner cell reactions and cell death.

MARCIA STAMELL, ASSOCIATE MANAGING EDITOR, marcia.stamell @photonics.com

Fluorescence lifetime imaging microscopy (FLIM) enables researchers in the life sciences to get information from live specimens about interactions on the molecular scale. The technique captures the differences in the excited state decay rate from a fluorescent sample, rather than relying on the concentration of a fluorophore. Since imaging does not derive from the intensity of a signal, the technique lessens the impact of photon scattering in thick layers of sample and is generally considered more robust than intensity-based methods. This can open doors for both researchers and clinicians. ...Read full article

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