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Fluorescence Lifetime Imaging: Choosing the Best Approach

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Fluorescence lifetime imaging microscopy (FLIM), which measures the spatial distribution of the fluorescence decay, can be achieved either in the time or in the frequency domain. Each approach exhibits advantages and disadvantages, depending on the type of light detector and acquisition method employed.

GERHARD HOLST, PCO AG

The term fluorescence is often applied as a synonym for photoluminescence, although luminescence actually covers fluorescence and phosphorescence. Both of these terms describe the process of absorption of light at a higher energy and subsequent re-emission at a lower energy. In this article, the...Read full article

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