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Fluorescence Microscopy: Getting the Picture Right

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Camera choice has to strike a balance between certain sensor properties, camera-related topics, and the needs of the intended applications in industry or medicine.

FELIX ASCHE, BASLER AG

Fluorescence microscopy is a very broadly used term covering numerous applications. It ranges from basic life science applications such as time-lapse cell viability assays — in which dead cells are counted as they start to fluoresce by intrusion of a fluorochrome that cannot enter intact living cells — to sophisticated techniques where only very few photons or single molecules are detected and localized by specific high-end hardware and software. It can be quite complex to design an optical imaging system that perfectly fits a specific application. And at the same time, instrument...Read full article

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