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Ghost Imaging Speeds Up Superresolution Microscopy

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Scientists from the Chinese Academy of Sciences have developed a new imaging technique that produces nm-scale resolution using significantly fewer images than traditional nanoscopy techniques. The scientists used ghost imaging to enhance the imaging speed of their technique. The new approach could be useful for live cell imaging. The technique is based on stochastic optical reconstruction microscopy (STORM), a wide-field microscopy method that uses fluorescent labels that switch between light-emitting (on) and dark (off) states. By acquiring hundreds or even thousands of images, each...Read full article

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    Published: January 2020
    Glossary
    superresolution
    Superresolution refers to the enhancement or improvement of the spatial resolution beyond the conventional limits imposed by the diffraction of light. In the context of imaging, it is a set of techniques and algorithms that aim to achieve higher resolution images than what is traditionally possible using standard imaging systems. In conventional optical microscopy, the resolution is limited by the diffraction of light, a phenomenon described by Ernst Abbe's diffraction limit. This limit sets a...
    nano
    An SI prefix meaning one billionth (10-9). Nano can also be used to indicate the study of atoms, molecules and other structures and particles on the nanometer scale. Nano-optics (also referred to as nanophotonics), for example, is the study of how light and light-matter interactions behave on the nanometer scale. See nanophotonics.
    stochastic optical reconstruction microscopy
    Stochastic optical reconstruction microscopy (STORM) is a super-resolution microscopy technique that enables imaging of biological specimens at resolutions beyond the diffraction limit of conventional optical microscopy. It falls under the category of single-molecule localization microscopy (SMLM) methods. STORM was first introduced in 2006 and has since become a powerful tool in biological research for visualizing fine details of cellular structures. The key principle behind STORM involves...
    fluorescence
    Fluorescence is a type of luminescence, which is the emission of light by a substance that has absorbed light or other electromagnetic radiation. Specifically, fluorescence involves the absorption of light at one wavelength and the subsequent re-emission of light at a longer wavelength. The emitted light occurs almost instantaneously and ceases when the excitation light source is removed. Key characteristics of fluorescence include: Excitation and emission wavelengths: Fluorescent materials...
    Research & TechnologyeducationAsia-PacificChinese Academy of SciencesImagingLight SourcesMicroscopyOpticsghost imagingcompressive imagingcomputational imagingsuperresolutionnanostochastic optical reconstruction microscopyfluorescencelive cell imagingBiophotonicscamerasnanoscopyBioScan

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