MUNICH, March 14, 2023 — Researchers at the Ludwig Maximilian University of Munich (LMU) combined pulsed-interleaved MINFLUX (pMINFLUX) nanoscopy with a newly developed method for axial resolution that exploits the special properties of the 2D material graphene to achieve 3D, superresolution imaging with nanometer-scale precision. The researchers combined the 2D localization of pMINFLUX with axial information from graphene energy transfer (GET) and with single-molecule switching using DNA point accumulation for imaging in nanoscale topography (DNA-PAINT). DNA-PAINT is a single-molecule localization microscopy...Read full articleRelated content from Photonics MediaARTICLESSuperresolution Method Could Improve Understanding of Gene FunctionAn interdisciplinary team from the Centre for Genomic Regulation (CRG) and the Institute for Research in Biomedicine (IRB Barcelona) has developed an imaging technique that captures the structure of...BioPhotonics Jan/Feb 2023 IssueTime-Tagging Captures Cellular Activity with Single-Photon MicroscopyBrightEyes-TTM, an open-source time-tagging module (TTM) developed at the Italian Institute of Technology (IIT), enables scientists to observe the dynamic processes of molecules inside living cells...Photonics.com 2/3/2023Internal Reflection-Based Microscopy Images Single ProteinA team from Arizona State University (ASU) has introduced evanescent scattering microscopy (ESM), a label-free method for sensitive imaging of biomolecules, including proteins. The single-molecule...Photonics.com 5/5/2022Nobel Prize-Winning Techniques Help Resolve Imaging ChallengeUsing a technique that was recently awarded the 2022 Nobel Prize in chemistry, researchers at Cornell University used expansion microscopy to study lipids, the water-repellent, dynamic components...BioPhotonics Jan/Feb 2023 IssuePRODUCTSFemtosecond Laser SourcesThe CRONUS-2P and CRONUS-3P microscopy-dedicated femtosecond laser sources from Light Conversion covers applications in functional neuroimaging, optogenetics, and deep imaging. CRONUS-3P...BioPhotonics May/Jun 2022 IssueLive-Cell ImagerThe CytoSMART Lux3 BR live-cell imager from CytoSMART Technologies BV is a small brightfield microscope equipped with a high-quality, 6.4-MP CMOS camera that allows researchers to perform long-term...BioPhotonics Nov/Dec 2021 IssueGlass-Bottom PlatesGlass Bottom µ-Plates from ibidi GmbH provide microscopy users with high-throughput possibilities for super-resolution and total internal reflection fluorescence. The µ-Plates have...BioPhotonics Mar/Apr 2023 IssueAutomated Cell CounterThe CytoSMART Exact FL automated cell counter from CytoSMART Technologies is a dual fluorescence device with an expanded field of view and high performance. The system features an advanced...BioPhotonics Jul/Aug 2021 IssuePHOTONICS HANDBOOK ARTICLESSuperresolution Microscopy: An Imaging Revolution: Despite tremendous accomplishments in recent years, superresolution microscopy is a relatively young field, and confusion exists in this domain of many abbreviations. However, this makes for captivating times as superresolution microscopy opens the door to wider imaging applications.Superresolution optical microscopy, for which the Nobel Prize was awarded to Eric Betzig, Stefan Hell, and William Moerner in 2014, has been one of the most momentous developments in the life...Photonics HandbookLasers for Microscopy: Major Trends: In order for biologists, drug developers, clinical lab professionals and other scientists to fully exploit new microscopy techniques and applications, parallel developments in laser technology are often required. Laser manufacturers are responding with products optimized to match the needs of these applications.Laser development for the microscopy market continues to be driven by key trends in applications, which currently include superresolution techniques, multiphoton applications in optogenetics and...Photonics HandbookFluorescence Lifetime Imaging: Choosing the Best Approach: Fluorescence lifetime imaging microscopy (FLIM), which measures the spatial distribution of the fluorescence decay, can be achieved either in the time or in the frequency domain. Each approach exhibits advantages and disadvantages, depending on the type of light detector and acquisition method employed.The term fluorescence is often applied as a synonym for photoluminescence, although luminescence actually covers fluorescence and phosphorescence. Both of these terms describe the process of...Photonics HandbookNanopositioning: A Step Ahead: It is almost a cliché among students of creativity that innovation occurs at the intersection of fields. Since nanopositioning is a discipline that has enabled swiftly evolving application arenas as diverse as materials science, genomics, photonics, defense, biophysics, and semiconductors, it’s unsurprising that its pace of innovation is nothing short of blistering in recent years.By its original definition, a nanopositioning device is a mechanism capable of repeatedly delivering motion in increments as small as one nanometer. Lately demands from industry and research have...Photonics HandbookWHITEPAPERSIs a DIY Microscope for you?Recently, it has become very popular for researchers to explore building their own microscopes. Budgetary and technical specifications are among the motivations for this approach. However, there is a...Photonics.com 10/1/20224 Steps to Optimize the Optics in Automated Imaging InstrumentsOptimize the performance and cost of your automated optical imaging system with 6 key equations for selecting an imaging sensor, objective lens, Z-focusing nano-positioning stage and XY sample...Photonics.com 5/17/2021Ingenuity + Instrumentation: Creating a Novel 4D Microscopy MethodTracking particles at the micro or nanoscale is inherently difficult due to the complexity of the systems studied, as well as the technical challenges: a blend of instrument design, photonics,...Photonics.com 9/12/2022Cutting Edge Measurement of an InsertThe 3D optical profilometer S neox Five Axis makes it possible to quickly and flexibly obtain the key parameters necessary for the continuous improvement of our tools, such as the cutting edge...Photonics.com 12/10/2021WEBINARSThe Future of Imaging: Three PerspectivesPresenters: Professor Stephen Boppart The Future of Healthcare With Optical Biomedical Imaging A medical doctor and professor at the University of Illinois, Boppart's...Photonics.com 12/14/2011Photonics for OphthalmologyPhotonics Media invites you to join us for our upcoming FREE digital conference, "Photonics for Ophthalmology." The event will feature several 15-minute online presentations on the use of light-based...Photonics.com 6/9/2016Sub-Cellular Biology at Tissue Scales with Cleared Tissue Axially Swept Light-Sheet MicroscopyLarge-scale interdisciplinary research efforts have worked to comprehensively catalog cellular architectures in health and disease. To aid in this effort, researchers have developed a scalable...Photonics.com 8/17/2022Quantum-Enhanced Superresolution Confocal MicroscopyFar-field optical microscopy beyond the Abbe diffraction limit is already a reality. Yet, while Abbe's theory was based on classical physics, quantum mechanical phenomena were only very recently used...Photonics.com 10/26/2021PHOTONICS DICTIONARY TERMSaxial vapor-phase depositionA vapor-phase oxidation process for fabricating graded-index optical fibers. It differs from outside vapor phase deposition in that the preform is developed radially rather than longitudinally. An...EDU.photonics.com 9/8/2009aberrationA departure from ideal paraxial imaging behavior. The distortion of an optical field wavefront as it is propagated through the elements of an optical system. The field distortion is due to the...EDU.photonics.com 1/6/2013interphako interference microscopyMeasures the refractive indices axially from the fiber profile. Microscopy technique provides an interferogram with high contrast with demonstrated improvements in relation to the Pluta two-beam...EDU.photonics.com 3/5/2018optical path difference (OPD)The relative path difference (or phase shift) traveled between two rays that pass through different mediums from the same object point. For a perfect optical system, the optical path, or distance,...EDU.photonics.com 3/5/2018MEDIASupporting the Photonics Industry from the Laboratory - LIGHT MATTERS 01.2018On this month's episode of Light Matters, we take you to central Massachusetts as Worcester Polytechnic Institute and Quinsigamond Community College take on a comprehensive joint photonics laboratory...Photonics.com 1/18/2018Capasso Group Revolutionizes Flat LensesResearchers at the Harvard John A. Paulson School of Engineering and Applied Sciences have developed a single flat lens that can focus the entire visible spectrum of light in the same spot and in...Photonics.com 7/5/2018Photonics on the Showroom Floor and in the Lab - LIGHT MATTERS 02.2018On this month's episode of Light Matters, we take you on a brief tour of the Higgins Laboratories at Worcester Polytechnic Institute (WPI). Quinsigamond Community College and WPI received a $4M grant...Photonics.com 2/21/2018Light Matters Extra: Taking Photonics Innovations from the Lab to the Operating Room - 01.2018In this month's Light Matters Extra, we give you an in-depth look at the relationship between researchers and scientists at the University of California Davis, and surgeons and clinicians at nearby...Photonics.com 1/18/2018PHOTONICS BUYERS' GUIDE CATEGORIESEpitaxial EquipmentCurrent MonitorsBorate CrystalsSuperresolution Imaging SystemsCOMPANIESAvantes BVAvantes has spent the last 30 years refining its mission as a trusted and innovative optical instruments and solutions source. Focused on applications and measurements, Avantes offers Spectrometers,...Photonics Buyers' GuideMad City Labs Inc.Mad City Labs, Inc. is a leading manufacturer of piezo nanopositioning systems and instrumentation capable of sub-nanometer positioning resolution. Our product line of nanopositioners,...Photonics Buyers' GuideOmega Optical LLCOmega Optical has been bringing you the light you need since 1969. Omega specializes in thin-film interference filters in the 190 nm - 10 µm wavelength range. The layers are...Photonics Buyers' GuideOptolong Optics Co. LtdOptolong Optics Co., Ltd is a manufacturer of fluorescence filters and astronomy filters. We also offer other interference filters such as UV filters, visible filters and near-infrared filters,...Photonics Buyers' Guide