PHOENIX, May 5, 2022 — A team from Arizona State University (ASU) has introduced evanescent scattering microscopy (ESM), a label-free method for sensitive imaging of biomolecules, including proteins. The single-molecule microscopy technique is based on total internal reflection (TIR), an optical phenomenon that occurs when light passes from a high-refractive medium, like glass, into a low-refractive medium, like water. ESM could allow researchers to visualize proteins and other biomolecules with more clarity and by simpler means than existing microscopy methods. TIR occurs when waves arriving at the...Read full articleRelated content from Photonics MediaARTICLESMicroscope Marks Head-Mounted Advance Toward Treating Neurological DisordersResearchers from the University of Colorado Boulder, the University of Colorado Anschutz Medical Campus, and Arizona State University have developed a head-mounted, lightweight, fluorescence...Photonics.com 4/8/2022Scientists Confirm Dynamin Molecular Motor’s Constriction MechanismA team at Kanzawa University used the measurement technique of single-molecule fluorescence resonance energy transfer, or smFRET, as well as a computable model, to develop a method that determined...Photonics.com 9/3/2021Fluorescent Sensors with Noncanonical Amino Acids Could Broaden Sensor Use in Biological StudiesAlthough fluorescence tools for biological study abound, some processes, such as the interactions between proteins and metabolites, are challenging to investigate with the tools that currently exist....Photonics.com 10/21/2021Three-Photon Microscopy Reveals Immune Cell Behavior in Real TimeA research group from Cornell University has used three-photon microscopy to image the full depth of the popliteal lymph node in a living mouse — reportedly for the first time. The researchers...BioPhotonics May/Jun 2022 IssuePRODUCTSField Emission Scanning Electron MicroscopeThe JSM-IT700HR InTouchScope field emission scanning electronic microscope from JEOL is a compact system that offers ultra-high resolution and versatile analytical capabilities. The JSM-IT700HR...Photonics.com 9/18/2020IR MicroscopeThe Nicolet RaptIR FTIR Microscope from Thermo Fisher Scientific Corp. is a Fourier Transform Infrared (FTIR) device that produces high-quality visual images for the drug discovery, design, and...Photonics Spectra May 2022 IssueMonochrome Microscope CameraThe DP23M Monochrome Microscope Camera from Olympus Corp. provides premium fluorescence images in a cost-effective package. The camera’s sensitive back-side illuminated monochrome CMOS...BioPhotonics Jan/Feb 2022 IssueLaboratory Reaction Support SystemThe ReactoMate DATUM from Asynt is a dual-rod stainless steel and aluminium support system built to ensure the stability and safety of lab reactors from 100 to 5000 mL. A neck clamp allows fast...Photonics.com 7/30/2020PHOTONICS HANDBOOK ARTICLESAspheric Lenses: Design Considerations: This first of two articles will cover design and manufacturing options for aspheres; the second will include more detail on manufacturing and testing processes. These articles will provide the tools optics designers need to tailor their designs to the appropriate manufacturing process and to optimize the cost of component fabrication.Aspheric surfaces are powerful tools that combine the optical corrections of multiple lenses into a single element (Figure 1) and affect performance in ways that spherical optics cannot. For example,...Photonics HandbookFluorescence Lifetime Imaging: Choosing the Best Approach: Fluorescence lifetime imaging microscopy (FLIM), which measures the spatial distribution of the fluorescence decay, can be achieved either in the time or in the frequency domain. Each approach exhibits advantages and disadvantages, depending on the type of light detector and acquisition method employed.The term fluorescence is often applied as a synonym for photoluminescence, although luminescence actually covers fluorescence and phosphorescence. Both of these terms describe the process of...Photonics HandbookMachine Vision Cameras: Making the Right Selection: Improvements and affordability have broadened the use of vision systems for inspection, but several factors should be considered before making a purchase.Technological advances have resulted in new, higher-quality sensors that enable machine vision cameras to deliver greater features and functionality at a lower cost than ever before. These factors...Photonics HandbookMicroscopy Illumination: Considering Nonlaser Light Sources: Metal halide and LED light sources are changing the illumination landscape in microscopy. Brighter, easier to use and growing in application, they are driving new options in fluorescence, conventional bright-field and other contrast techniques.As recently as five years ago, shopping for a microscope light source was fairly straightforward. For routine applications, it was tungsten halogen, typically 100 W, while for fluorescence, it was an...Photonics HandbookWHITEPAPERSHigh-Speed Imaging in Biomedical Microfluidic ResearchThis whitepaper discusses multiple case studies when Phantom cameras have assisted in the advancement of microfluidic and cellular research.Photonics.com 11/17/2020A 0.19 e- rms Read Noise 16.7 Mpixel Stacked Quanta Image Sensor with 1.1 µm-Pitch Backside Illuminated PixelsThis paper reports a 16.7 Mpixel, 3D-stacked backside illuminated Quanta Image Sensor (QIS) with 1.1 µm-pitch pixels which achieves 0.19 e- rms array read noise and 0.12 e- rms best single-pixel read...Photonics.com 2/11/2022Advantages of Measuring Surface Roughness with White Light InterferometryThis application note discusses the use of mean roughness measurements with white light interferometry (WLI) optical profilers. 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To fully understand brain function, it is necessary to map out multiple cell types and subcellular features (e.g., dendrites and axons) and probe functional...Photonics.com 10/26/2021Photonics Spectra Spectroscopy Conference 2022: April 12 - 13Discover the latest trends in spectroscopy. Join us April 12-13, 2022 at the Photonics Spectra Spectroscopy Conference! The publishers of Photonics Spectra invite you to attend a free virtual...Photonics.com 4/12/2022Label-Free Imaging of Drugs and Tissues in Biomedicine Using Pharmacokinetic Tomography and Stimulated Raman Scattering MicroscopyConor Evans introduces pharmacokinetic (PK) tomography — 3D quantitative imaging of drug uptake within tissue — using stimulated Raman scattering microscopy. He presents machine...Photonics.com 4/13/2022PHOTONICS DICTIONARY TERMSdifferential interference contrast microscopyDifferential interference contrast (DIC) microscopy, sometimes called Nomarski Microscopy after the inventor Georges Nomarski, is one of several methods used to obtain microscope images of...EDU.photonics.com 3/5/2018surface electromagnetic waves (SEW)Waves that propagate along the interface between two different media without radiation with exponentially decaying evanescent fields on both sides.EDU.photonics.com 9/8/2009evanescent field theoryA high-frequency approach to the propagation of light in graded-index fibers in which the modal field is represented in terms of an inhomogeneous (evanescent) plane wave with a complex phase, so that...EDU.photonics.com 9/8/2009axial gradientIn gradient index optics, a gradient profile in which the refractive index varies along the direction of light propagation, or optical axis. Axial gradient lenses can be used to correct spherical...EDU.photonics.com 9/8/2009MEDIAPhotonics on the Showroom Floor and in the Lab - LIGHT MATTERS 02.2018On this month's episode of Light Matters, we take you on a brief tour of the Higgins Laboratories at Worcester Polytechnic Institute (WPI). Quinsigamond Community College and WPI received a $4M grant...Photonics.com 2/21/2018Supporting the Photonics Industry from the Laboratory - LIGHT MATTERS 01.2018On this month's episode of Light Matters, we take you to central Massachusetts as Worcester Polytechnic Institute and Quinsigamond Community College take on a comprehensive joint photonics laboratory...Photonics.com 1/18/2018Light Matters Extra: Taking Photonics Innovations from the Lab to the Operating Room - 01.2018In this month's Light Matters Extra, we give you an in-depth look at the relationship between researchers and scientists at the University of California Davis, and surgeons and clinicians at nearby...Photonics.com 1/18/2018Light Matters Extra: Weird and Strange: Quantum Photonics - 03.2018In this month's Light Matters Extra, we take you to the Quantum Photonics Laboratory at MIT run by Dr. Dirk Englund. Dr. Englund's group, the Quantum Photonics Group, is made up of postdocs and...Photonics.com 3/29/2018PHOTONICS BUYERS' GUIDE CATEGORIESMicroscopy Immersion LiquidsGRIN LensesMicroscope LensesCell Assay Imaging SystemsCOMPANIESLaCroix Precision OpticsOptics that Defend, Build, Cure and Discover The LaCroix name is synonymous with quality precision optics. Since 1947, three generations of family leadership have placed LaCroix Precision Optics...Photonics Buyers' GuideOptolong Optics Co. LtdOptolong Optics Co., Ltd is a manufacturer of fluorescence filters and astronomy filters. We also offer other interference filters such as UV filters, visible filters and near-infrared filters,...Photonics Buyers' GuideFOCtek Photonics Inc.FOCtek provides customers with all types of custom manufactured lenses, Precision Optical Components, Polarizing Optics, Prisms, Filters, Coatings, Optical Systems, Crystals, Nonlinear Crystals,...Photonics Buyers' GuideDiverse Optics Inc.Diverse Optics transforms optical systems from expensive, heavy, and complicated to commercially viable, lightweight, and simple. We manufacture custom precision polymer optic components and...Photonics Buyers' Guide