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Lasers for Microscopy: Major Trends

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Marco Arrigoni, Nigel Gallaher, Darryl McCoy, Volker Pfeufer and Matthais Schulze, Coherent Inc.

Laser development for the microscopy market continues to be driven by key trends in applications, which currently include superresolution techniques, multiphoton applications in optogenetics and other areas of neuroscience, and even a shift in multiphoton imaging toward preclinical and clinical usage. In spite of its long history, optical microscopy – particularly laser-based microscopy – is a very dynamic field. New techniques continue to be developed, while existing techniques are being applied to new applications. For biologists, drug developers, clinical lab...Read full article

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    Published: April 2015
    Glossary
    superresolution
    Superresolution refers to the enhancement or improvement of the spatial resolution beyond the conventional limits imposed by the diffraction of light. In the context of imaging, it is a set of techniques and algorithms that aim to achieve higher resolution images than what is traditionally possible using standard imaging systems. In conventional optical microscopy, the resolution is limited by the diffraction of light, a phenomenon described by Ernst Abbe's diffraction limit. This limit sets a...
    optogenetics
    A discipline that combines optics and genetics to enable the use of light to stimulate and control cells in living tissue, typically neurons, which have been genetically modified to respond to light. Only the cells that have been modified to include light-sensitive proteins will be under control of the light. The ability to selectively target cells gives researchers precise control. Using light to control the excitation, inhibition and signaling pathways of specific cells or groups of...
    camerascontinuous wave lasersFeaturesfiber laserssemiconductor laserssuperresolutionTunable LasersBiophotonicsMicroscopyLasersImagingEuropeAsia-Pacificlaser-based microscopySTEDsuperresolution microscopynanoscopyCW lasersEric BetzigStefan HellWilliam Moerneroptical switchingfluorescent labelsRESOLFT microscopySIMd-STORMf-PALMoptically pumped semiconductor laserOPSLObis FP lasermultiphoton imagingMPEmultiphoton excitationultrafast lasersoptogeneticslaser manufacturingCoherent

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