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Microscopy Techniques Combine for Nanoscale Brain-Wide Imaging

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Scientists from the Eric Betzig lab at Howard Hughes Medical Institute’s Janelia Campus and the Ed Boyden Lab at Massachusetts Institute of Technology (MIT) collaborated to develop an imaging technique, ExLLSM, that combines expansion microscopy (ExM) with lattice light-sheet microscopy (LLSM) for nanoscale imaging of fly and mouse neuronal circuits at the molecular level. ExLLSM images neural structures with molecular contrast over millimeter-scale volumes, including (clockwise from top right) mouse pyramidal neurons and their processes; organelle morphologies in somata;...Read full article

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